Analysis of Polymorphism of TLR2 Gene and the Relationship between the Gene and Mastitis Resistance in Chinese Holstein Cattle

doi:10.7668/hbnxb.2008.06.021

Abstract

Abstract: In this research,polymorphisms ofTLR2gene in Chinese Holstein(n=297)were detected with PCR-RFLP,and relationship between polymorphisms of TLR2 with somatic cell score(SCS)was analyzed.Results showed that a polymorphic site was identified,where there was a mutation C→A at position 106 bp after sequencing,which resulted in Asp replaced by Glu.Genotype AA,AB and BB were detected by digesting the fragment with restriction endonucLeaseEcoR V in the population.B allele was predominant and allele frequency A and B were estimated as 0.784 5,0.215 5.χ²test indicated that the polymorphism locus in Chinese Holstein fitted Hardy-Weinberg equilibrium(p<0.05).In addition,the effect of theTLR2polymorphism on SCS was analyzed.Results indicated that the SCS of BB and AB genotype cows were significantly higher than AA genotype(p<0.05).AA genotype was beneficial to mastitis resistance.We concluded thatTLR2gene could be candidate gene of mastitis resistance for molecule marker-assisted selection breeding.This study might offer a theoretical basis on selection of the mastitis resistance in Holstein cattle.

Key words: TLR2, PCR-RFLP, Somatic cell score, Mastitis resistance, Chinese Holstein cattle

CLC Number:

S823

ZHANG Cui-xia, WANG Hong-mei, LI Jian-bin, WANG Chang-fa, LAI Song-jia, LI Qiu-ling, ZHONG Ji-feng. Analysis of Polymorphism of TLR2 Gene and the Relationship between the Gene and Mastitis Resistance in Chinese Holstein Cattle[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 93-96. doi: 10.7668/hbnxb.2008.06.021.

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