Isolation and Identification of Bacillus thuringiensis Strains Containing cry8 Genes

doi:10.7668/hbnxb.2014.06.026

Abstract

Abstract: It is very important to explore the source of both Bacillus thuringiensis and its cry gene for pest control.In this study,using a method that the soil suspensions were incubated at 80 ℃ for 20 min in a water bath,forty two Bt strains were isolated from 254 soil samples which were collected from different counties of Hebei province.Ten strains of them are identified that they contain the different plasmids harboring cry8 gene.PCR-RFLP analysis revealed that:One strain may have 1 or more kinds of plasmids;PCR-RFLP results showed that there were multiple cry8-type genes in the strains and one strain usually contained two subtypes of cry8 gene,for example, cry8E and cry8F which were often coexist in the same strain;The Cry8-type insecticidal crystal proteins has a molecular weight around 130-140 kDa with spherical parasporal crystals in shape.The soil samples containing the cry8-type Bt strains were collected from Baoding,Hengshui,Handan,Langfang,Tangshan and Xingtai areas of Hebei province with various vegetation.These results implied that the cry8-type Bt strains extensively distribute in Hebei province and may provide abundant resources of Bt with insecticidal activity against the cockchafer pests.

Key words: Bacillus thuringiensis, Gene identification, cry8 gene, PCR-RFLP, Cockchafer

CLC Number:

S432

WANG Zhi-xin, SHU Chang-long, SHEN Pei-li, SU Xu-dong, LI Zhi-hui, YU Yan, ZHANG Xian-zhou, MA Wen, TAN Jian-xin. Isolation and Identification of Bacillus thuringiensis Strains Containing cry8 Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 149-154. doi: 10.7668/hbnxb.2014.06.026.

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