Isolation and Identification of Bacillus thuringiensis Strains Toxic to Scarabaeoidae Larvae

doi:10.7668/hbnxb.2008.06.018

Abstract

Abstract: In order to finding new Bt resource,two hundreds and eighty-fiveBacillus thuringiensiswere isolated from three different soil-type samples.Thirteen of them were shown to be highly toxic to scarabaeoidae larvae.By using of the PCR-RFLP system and SDS-PAGE,the types of gene cry of the 13Bacillus thuringiensisisolates and the expression of insecticidal crystal proteins were analyzed.The result is that twelve of them have thecry8Cagene which coding the crystal proteins kill Scarabaeoidae larvae.FTL53 couldn't be finded any gene.The thirteen Bt strains express 130 kDa protein.This research lays a foundation to find new and high toxin cry gene.

Key words: Bacillus thuringiensis, PCR-RFLP, Scarabaeoidae larvae

CLC Number:

S476.11

HU Xiao-ting, SONG Jian, WANG Rong-yan, DU Li-xin, CAO Wei-ping, WANG Jin-yao, FENG Shu-liang. Isolation and Identification of Bacillus thuringiensis Strains Toxic to Scarabaeoidae Larvae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 81-83. doi: 10.7668/hbnxb.2008.06.018.

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http://www.hbnxb.net/EN/Y2008/V23/I6/81

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