Analysis of SNPs in Promoter Region of Myostatin in Sheep

doi:10.3969/j.issn.1000-7091.2012.03.016

Abstract

Abstract: Sequencing along with PCR-RFLP was applied to analyze the polymorphism of MSTN gene promoter?in seven sheep breeds. Two SNPs at －959(T/C)and －784(G/A) including six genotype (TT，TC，CC，GG，GA，?AA)respectively were detected in the promoter region，At －959(T/C)，the predominant genotype of TT was identified in Dorset，German Merino and Bashibai sheep，the CC in Bayinbuluke，Duolang and Aletai sheep. A chi-square?analysis suggested that the allele frequencies and genotype frequencies of two polymorphisms were in Hardy-Weinberg equilibrium. Statistical analysis also revealed the variation of intermediate polymorphism information content?(PIC) in six breeds (0. 25 ＜ PIC ＜0. 50). At －784(G/A)，The G was predominant allele in all breeds，whereas?the low polymorphism information content (PIC) was observed in German Meilin and Aletai populations (PIC ＜?0. 25) and intermediate PIC in the Bayinbuluke，Duolang and Bashibai populations (0. 25 ＜ PIC ＜0. 50).

Key words: Sheep, MSTN, Promoter, PCR-RFLP, Polymorphsim

CLC Number:

HE San-gang, CHEN Xin, LIU Chen-xi, LIU Guo-song, LIU Ming-jun. Analysis of SNPs in Promoter Region of Myostatin in Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 81-85. doi: 10.3969/j.issn.1000-7091.2012.03.016.

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