Isolation of IGFBP1 and SNP Loci Related to Body Weight Gain in Cyprinus carpio var. jian

doi:10.3969/j.issn.1000-7091.2012.03.015

Abstract

Abstract: Two IGFBP1 genes were isolated from Cyprinus carpio var. jian. Seven and twenty-four SNP loci were?identified on IGFBP1a and IGFBP1b，respectively. There are two and four SNPs were located at exons of 1a and?1b，respectively. PCR-RFLP was applied to detect genotypes of 913 individuals (♂469，♀444) at two SNPs (1b?exon 1_A210G，1b exon 3_C108T). Correlation analysis between genotypes and weight gain showed:both two SNPs?were associated with male weight gain significantly (P＜0. 01). To these two SNPs loci，GG and CC genotype individuals grew faster than other individuals in the juvenile stage and it was AG and CC genotype in the adult stage.?A210G was also significantly related to female juvenile weight gain (P ＜0. 01) and AG individuals grew significantly faster than AA ones. The CC and CT type male adult weight gains of C108T were significantly (P＜0. 01)?larger than TT type. Nine diplotypes were found in this experimental population. Multiple comparison results displayed that the body weight gains of D4，D5，D7 were significantly larger than those of D6，D9. D4，D5 and D7 individuals accounted for sixty percent in experimental population，so there still are spaces for further improved. A210G and C108T identified in this experiment can be used in molecular breeding of Cyprinus carpio var. jian.

Key words: Cyprinus carpio var jian, Insulin-like growth binding protein 1, Single nucleotide polymorphism, Weight gain, Correlation

CLC Number:

Q953
Q78

WEI Ke-peng, YU Ju-hua, LI Hong-xia, LI Jian-lin, TANG Yong-kai, XIA Zheng-long. Isolation of IGFBP1 and SNP Loci Related to Body Weight Gain in Cyprinus carpio var. jian[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 75-80. doi: 10.3969/j.issn.1000-7091.2012.03.015.

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