The Interaction of MC4R with Leptin，MC3R and CCKAR Genes

doi:10.3969/j.issn.1000-7091.2012.03.014

Abstract

Abstract: To study the potential relationships among the four genes associated with intake，recombinant method?was used to build a Melanocortin receptor-4 (MC4R) gene eukaryotic expression vector. The expression vector was?transfected into fibroblasts by liposome method. Transient transfection after 24 h，Real-time PCR method was used to?detect and analyze Leptin，MC3R and CCKAR mRNA expression changes. The results showed that，in fibroblasts，?overexpression of MC4R gene，Leptin，MC3R and CCKAR mRNA levels were not significantly changed. Presumed?that although these four genes are all involved in the regulation of animal feed intake，but they are likely to be regulated by a different pathway.

Key words: Pig, Fibroblasts, MC4R, Real-time PCR, Overexpression

CLC Number:

ZHANG Dong-jie, WANG Liang, LIU Di. The Interaction of MC4R with Leptin，MC3R and CCKAR Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 72-74. doi: 10.3969/j.issn.1000-7091.2012.03.014.

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