Construction and EST Analysis of a Normalized Full-Length cDNA Library from Water Stressed Korshinsk Peashrub Leaves

doi:10.3969/j.issn.1000-7091.2012.03.013

Abstract

Abstract: In order to study the drought tollerence genes of korshinsk peashrub，a normalized full-length cDNA?library was constructed using Creator SMART technology combined with duplex-specific nuclease(DSN) from water?stressed leaves. The titer of original library was 1. 5 ×106cfu/mL，the recombinant efficiency was 93. 75% and the?average of insert size was over 1 kb，these results showed the cDNA library was of high quality. Besides，3 200?clones from it were selected randomly and sequenced. 2 429 unigenes were obtained，among them 2 229 was singlets，means this library was normalized well. Some genes which response to drought were found after BLAST.

Key words: Korshinsk peashrub, Water stress, Normalized full-length cDNA library, EST analysis

CLC Number:

S793.3

GUO Jiu-feng, SUN Guo-qin, QIAO Hui-lei. Construction and EST Analysis of a Normalized Full-Length cDNA Library from Water Stressed Korshinsk Peashrub Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 67-71. doi: 10.3969/j.issn.1000-7091.2012.03.013.

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