Expression Analysis of PpKO Gene in Pear Shoots Apical Tissue by Real-time Fluorescent Quantitative PCR

doi:10.3969/j.issn.1000-7091.2012.03.012

Abstract

Abstract: The expression stability of 4 normal used reference genes，18S rRNA，Actin，GAPDH and S19，in pear?shoot apical tissue at different developmental period was evaluated by real-time fluorescent quantitative PCR technique. GeNorm software analysis indicated that the value of average expression stability (M) was 0. 365，0. 392，?0. 457 and 0. 517，respectively，which meant the sequence of expression stability from higher to lower was Actin ＞?18S rRNA ＞ GADPH ＞ S19. Finally，Actin was selected as the reference gene for assaying ent-kaurene oxidase gene?PpKO expression in the apical tissue of pear shoots. The result showed that there were two expression peaks in the?procedure of shoots development under growing season coinciding with the shoots primary and secondary rapid?growth period. Moreover，the expression was much higher in the secondary shoots than the primary shoots.

Key words: Pear, Reference gene, Ent-kaurene oxidase gene (PpKO), Quantitative expression

CLC Number:

TIAN Yi-ke, LI Jie-fa, WANG Cai-hong, YIN Hao, BAI Mu-dan. Expression Analysis of PpKO Gene in Pear Shoots Apical Tissue by Real-time Fluorescent Quantitative PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 62-66. doi: 10.3969/j.issn.1000-7091.2012.03.012.

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