Effects of Exogenous Spd on the Pollen Tube Growth and Stylar Protein Expression During Pollination and Fertilization of Yali Pear

doi:10.7668/hbnxb.2016.01.004

Abstract

Abstract: Yali pear belongs to the kind of gametophytic self-incompatibility,the percentage of self-pollinated fruit set is extremely low.Our previous research showed that sprayed polyamine in a concentration range at florescence could break self-incompatibility of Yali pear.In order to explore the physiological and biochemical mechanism,the effects of exogenous Spd on the pollen tube growth and stylar protein expression of the Yali pear were studied by two-dimensional gel electrophoresis and mass spectrum analysis technology.The main results were as follows:The pollen tube length were extremely significantly increased after suitable concentration of Spd treatment,the best effects of exogenous Spd were 0.25 mmol/L Spd treatment,the number of pollen tube reached to the bottom of style were the most after 72 hours of treatment.Three specific proteins were found in the electrophoretogram.Two protein spots were obtained by the mass spectrometry results,which were predicted protein and putative protein respectively.

Key words: Spermidine, Yali pear, Pollen tube, Stylar protein

CLC Number:

Q78
S661.03

YUE Lei, LI Zhongyong, YIN Baoying, ZHANG Yuan, XU Jizhong. Effects of Exogenous Spd on the Pollen Tube Growth and Stylar Protein Expression During Pollination and Fertilization of Yali Pear[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 22-28. doi: 10.7668/hbnxb.2016.01.004.

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