Abstract: To study the function of lipid transfer proteins(LTP)in abiotic stress,the ZmLTP3 gene was transferred into maize Jing 2416 inbred lines by pollen tube pathway method. Positive transgenic lines were obtained by continuous selfing based on the screening of PCR and EPSPS. Three transgenic lines OE6,OE10 and OE18 were selected as the experimental group,and Jing 2416 inbred lines(WT)were used as CK. Both of them were subjected to salt stress. Morphological and physiological indexes were compared between OE and WT lines. The results showed that OE and WT plants showed no significant differences in both morphological and physiological indexes under normal condition. Under the salt stress condition,compared with WT plants,the OE plants had significantly higher height,stem diameter,root length,fresh weight,dry weight and chlorophyll content,significantly higher activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),significantly lower malondialdehyde(MDA)content and relative conductivity ratio level,showing their better growth status,higher active oxygen radicals scavenging capacity and less cell injury. These results indicated that overexpression of ZmLTP3 gene could improve the salt tolerance in transgenic maize.

Key words: ZmLTP3 gene, Pollen tube pathway method, Transgenic maize, Salt tolerance

CLC Number: 

• Q78

• S513.03