Abstract: In order to further study the Na⁺/H⁺ antiporter roles of vacuole membrane in salt tolerance of plants,the salt-tolerant tartary buckwheat variety Chuanqiao No.1 was used as materials,and the NHX gene was obtained by homology cloning,which named FtNHX1,and registered in GenBank,the landing number was KY438929. Sequence analysis showed that the open reading frame of FtNHX1 was 1 662 bp,encoding 553 amino acids,with predicted molecular weight of 61.24 kDa and isoelectric point of 5.15. Phylogenetic tree analysis showed that FtNHX1 was closely related to AtNHX1,OsNHX1 and TaNHX1,with 60.22%,58.95% and 57.30% amino acid homology rates. Quantitative real-time PCR analysis showed that the relative expression of FtNHX1 gene in roots,stem base and leaf of tartary buckwheat was significantly increased with the concentration increasing of NaCl stress,and that increased the most under NaCl stress of 150 mmol/L,which was increased by 254.10%,311.35% and 256.18% respectively in contrast with control. The average expression of FtNHX1 gene in roots and stem base and leaf of tartary buckwheat was increased by 109.46%,145.67% and 155.94% in contrast with control under NaCl stress,and that in stem base and leaf was very higher,indicating that the expression of FtNHX1 gene in tartary buckwheat was obviously induced and regulated by salt stress,and there was a close relationship between the FtNHX1 gene and the salt tolerance of tartary buckwheat.

Key words: Tartary buckwheat, Salt stress, Gene cloning, Relative expression, Salt tolerance

CLC Number: 

• Q78

• S517