Meta-analysis of Salt Tolerance QTLs and Mining of Candidate Genes

doi:10.7668/hbnxb.2017.06.007

Abstract

Abstract: In order to obtain the effective information of QTLs related to salt tolerance in rice from previous studies,the purpose of mining candidate genes in interval of QTLs was realized.Six rice genetic maps were integrated and 344 QTLs related to salt tolerance of rice were collected.The collected QTLs were analyzed by BioMercator V4.2 software,and then the bioinformatics analysis was performed on the QTLs of the consistent QTLs.The results showed that 46 QTLs related to salt tolerance of rice were detected.8 QTLs with physical distance close to 0.5 Mb were analyzed by physical map and candidate gene analysis.A total of 1 489 genes were found,involved in cell synthesis,catalytic activity,hydrolase activity and metabolism and other major functions.9 rice salt tolerance candidate genes were discovered by comparatively analyzing the salt tolerance gene from the consensus QTL regio n,those 9 candidate genes (OS01G0813100,OS01G0869900,OS02G0753000,OS03G0282100,OS03G0607700,OS07G0150500,OS09G0369050,OS09G0434500 and OS09G0522100) have the same conserved domain with salt tolerance gene.The results showed that the markers with Mqtl at the genetic distance of less than 0.1 cM could be used for the molecular marker-assisted breeding;9 annotated genes with high protein sequence similarity to salt tolerance genes in rice were found.These candidate genes could lay the foundation for cloning and functional verification of salt tolerance genes in rice.

Key words: Rice, Traits of salt tolerance, Meta analysis, Candidate genes

CLC Number:

LEI Lei, ZHENG Hongliang, YANG Luomiao, ZHAO Hongwei, WANG Jingguo, LIU Hualong, SUN Jian, ZOU Detang. Meta-analysis of Salt Tolerance QTLs and Mining of Candidate Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 45-53. doi: 10.7668/hbnxb.2017.06.007.

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