Abstract: In order to explore the genetically modified effects of excessive expression of superoxide dismutase in the upland cotton.Cotton cytoplasmic superoxide dismutase gene sequences of nucleic acids was found in the Genbank database,a pair of specific primers were designed,the cotton cytoplasmic superoxide dismutase gene was cloned and the plant binary expression vector was built,the excessive expression of transgenic cotton plants were obtained by used a new technology of cotyledon axillary bud of Agrobacterium mediated genetic transformation.PCR and Southern Blot analysis indicated that the new copy Cu/Zn-SOD gene had been integrated into the genome of cotton.Germination experiment of genetically modified materials T₁ generation seeds showed that under the stress of NaCl,the average length of taproot,bud average long and average number of lateral root of genetically modified materials were greater than the negative control,the SOD activity in transgenic plants leaves were significantly improved than that in the control,so were the chlorophyll contents,the MDA content was dramatically declined,but praline contents was no changed.If excessive cytoplasmic SOD genes were expressed in the cotton,the SOD activity of plantlet can be increased,this can reduce salt stress caused by the cell membrane peroxidation,and enhance the ability of plants to resist salt stress.

Key words: Cotton, Superoxide dismutase gene, Genetic transformation, Salt tolerance ability

CLC Number: 

• Q78

• S562.03