Expression Vector Construction,Transformation and Cold Resistance of GhSAD2 Gene from Upland Cotton

doi:10.7668/hbnxb.2017.06.009

Abstract

Abstract: In order to study a △9-stearoyl-ACP desaturase(GhSAD2)gene function and its correlation with the cold resistance of plants, GhSAD2 gene in Upland Cotton (KX197920) gene and construct the plant expression vector pBI121-GhSAD2,the expression vector by electroporation into Agrobacterium GV3101,tobacco leaf disc method by Agrobacterium transformation tumefaciens. Identification of exogenous GhSAD2 gene was transferred and integrated and integrated into the tobacco genome by PCR expression and GUS activity staining. The pBI121 -GhSAD2 type and wild type tobacco at 48 h treatment at 4℃,the detection rate of electrolyte leakage and to observe the phenotype. The results proved that the GhSAD2 gene had been integrated into the genome of tobacco and cotton,received a total of 9 transgenic plants,the conversion rate of 75% in Upland Cotton GhSAD2 gene could significantly enhance the cold resistance and cold acclimation non tobacco,so as to improve the low temperature the defense ability of plant cells,identification of the foundation for further research of the gene function.

Key words: Gossypium hirsutum L., GhSAD2 gene, Expression vector construction, Genetic transformation

CLC Number:

Q78
S562.03

CAI Man, LI Weihua, LIU Yantao, YU Yu, KONG Xianhui, WANG Juan, WANG Xuwen, LIU Li. Expression Vector Construction,Transformation and Cold Resistance of GhSAD2 Gene from Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 60-66. doi: 10.7668/hbnxb.2017.06.009.

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