Genetic Transformation of ACO Antisense Gene into Dendrobium officinale Kimura et Migo. by Agrobacterium Mediation

doi:10.7668/hbnxb.2015.02.004

Abstract

Abstract: The ACO antisense gene was transformed into the protocorms of Dendrobium officinale by Agrobacterium mediation.To optimize the transformation efficiency, several related factors were studied in this paper.The results showed that, after infected for 30 min at 100 μmol/L of AS, 0.8 of OD₆₀₀and 60 h of incubation in darkness, the cut protocorms gained the highest transformation rate in the medium of 30 mg/L Mer and 3.0 mg/L PPT.The selected plants were tested by PCR and GUS histochemical staining, indicating that the ACO antisense gene had been successfully integrated into D.officinale.

Key words: Dendrobium officinale, ACC oxidase, Genetic transformation, Agrobacterium tumefaciens

CLC Number:

WU Rong-hua, KANG Ying-ying, WANG Jie-qiong, LIU Jia, CUI Bo, JIANG Su-hua, ZHANG Kai-ming. Genetic Transformation of ACO Antisense Gene into Dendrobium officinale Kimura et Migo. by Agrobacterium Mediation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 17-21. doi: 10.7668/hbnxb.2015.02.004.

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