Cloning,Bioinformatic Analysis and Expression Analysis of APX Gene from Ipomoea trifida (2X)

doi:10.7668/hbnxb.2015.02.003

Abstract

Abstract: In order to get stress resistance genes from wild resources of sweet potato, total RNA was extracted from tissue culture seedlings of Ipomoea trifida, cDNA first-strand was obtained by reverse transcription, a 769 bp sequence was amplified by PCR using specific primers.The I.trifida ascorbate peroxidase enzyme coding region was obtained, bioinformatic analysis and expression pattern analysis were conducted.Sequencing results showed that the sequence contained a complete open reading frame of 753 bp encoding 250 amino acid residues.The putative protein size was approximately 27.6 kDa, isoelectric point was 5.42, and there was a wide range of hydrophilic region.It was speculated that the protein played antioxidant role in the cytoplasm.Phylogenetic analysis indicated that IbAPX was most closely genetically related to ItAPX.Tissue-specific expression analysis showed that ItAPX expressed in all organs.Cloning of I.trifida ascorbate peroxidase gene would help sweet potato breeding for stress-tolerance with new genetic resources.

Key words: Sweet potato, Ipomoea trifida, APX, Anti-oxidation

CLC Number:

S531.03
Q78

ZHANG An, CAO Qing-he, ZHOU Zhi-lin, ZHAO Dong-lan, PANG Hong-bo, TANG Jun. Cloning,Bioinformatic Analysis and Expression Analysis of APX Gene from Ipomoea trifida (2X)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 12-16. doi: 10.7668/hbnxb.2015.02.003.

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