Inhibition of Strawberry FaEtr2 Gene Expression by RNA Interfering

doi:10.7668/hbnxb.2014.01.006

Abstract

Abstract: In order to delay the ripening and softening process of strawberry,RNA interference technology was used to inhibit strawberry ethylene receptor gene expression. Two pairs of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid. PCR products and the plasmid pBI121 were digested by the corresponding restricted enzymes respectively,and linked directionally. Then the shRNA expression vector can be designed. After transcription,the hairpin mRNA obtained due to the inverted-repeat DNA fragment. The constructed expression vector was transformed into Agrobacterium LBA4404 for the following-up genetic transformation research. Four strains of transgenic plants were detected by PCR and GUS histochemical staining.

Key words: Strawberry, FaEtr2 gene, RNAi, Genetic transformation

CLC Number:

SONG Chun-li, MA Jun-lian, TANG Xia, ZHANG Zi-de, ZHAO Cong-zhi, LI Jing. Inhibition of Strawberry FaEtr2 Gene Expression by RNA Interfering[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 28-30. doi: 10.7668/hbnxb.2014.01.006.

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