Cloning and Expression Analysis of MbFAD2 Gene in Malus baccata

doi:10.7668/hbnxb.2014.01.007

Abstract

Abstract: The cDNA sequence of MbFAD2 was cloned from Malus baccata by RT-PCR. It's open reading frame possesses 1 149 bp,and encodes a peptide of 382 amino acids. It has a calculated molecular mass of 97. 511 2 kDa and a theoretical PI of 5. 01. The gene was named as MbFAD2,and its accession nucleotide sequence number in GenBank was KC702671. Secondary structure analysis showed that MbFAD2 protein contains 35. 86% α-helical domains,16. 23% extended strand,and 47. 91% random coil. Phylogenetic tree analyses showed that MbFAD2 is closest to the Populus tomentosa and Hevea brasiliensis,the farthest is Caragana korshinskii. The expression of MbFAD2 was determined by real-time quantitative RT-PCR. The result showed than the MbFAD2 was expressed in different tissue organs. The highest mRNA expression was found in flower,secondly is leaf. The MbFAD2 expression could be significantly induced by low temperature(4 ℃) for 24 h,but the stem is difference. These results suggest that MbFAD2 may be involved in low temperature.

Key words: Malus baccata, MbFAD2, Low temperature treatment, Expression analysis

CLC Number:

LIU Xiu-li, LU Jing-jing, ZHOU Ai-qin, ZHANG Yu-gang, ZHU Jun, DAI Hong-yi. Cloning and Expression Analysis of MbFAD2 Gene in Malus baccata[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 31-35. doi: 10.7668/hbnxb.2014.01.007.

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