Construction of Plant Overexpression Vector of Wheat TaNADP-ME1 Gene and Genetic Transformation into Rice

doi:10.7668/hbnxb.2014.01.005

Abstract

Abstract: The wheat TaNADP-ME1 gene was responsive to drought,salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology,transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method,acquired resistant callus by hygromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were acquired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.

Key words: TaNADP-MEl, Transformation, Rice

CLC Number:

FU Zhen-yan, LIU Feng, GOU Xiao-qing, WANG Xiao-jun. Construction of Plant Overexpression Vector of Wheat TaNADP-ME1 Gene and Genetic Transformation into Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 25-27. doi: 10.7668/hbnxb.2014.01.005.

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http://www.hbnxb.net/EN/Y2014/V29/I1/25

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