Abstract: Aphid transmission of the virus as a mediator harm plants far more than economic losses caused by aphids themselves. So,rapid,sensitive and specific method,by which viruses carried by aphids can be detected simultaneously,is needed urgently. In this study,according to the Lily symptomless virus(LSV) and the Tobacco mosaic virus(TMV) coat protein gene sequence,primers and probes were designed,gene chip was prepared. The downstream primers were labeled by Cy3. The total RNA was extracted from Aphis gossypii and Myzus perdicae raised with lily infected by LSV and TMV respectively. The product amplified by RT-PCR using the labeled primers was hybridized with the gene chip. The hybridization signal was detected and analyzed by a fluorescence scanner. The result showed that the chip can simultaneously detect both aphids infected cases,this method has good specificity and reproducibility,fast and accurate on the mediator infected aphids were detected.

Key words: Aphais gossypii, Myzus perdicae, Lily symptomless virus, Tobacco mosaic virus, Gene, Detection

CLC Number: 

• S432.4

• Q78