Proteomics Analysis of Mechanism of 6-benzyl Adenine Deferring Contabescence of Rice

doi:10.7668/hbnxb.2014.01.003

Abstract

Abstract: In order to reveal the mechanism of 6-benzyl adenine deferring contabescence of rice,the hybrid rice Weiyou 916 seedlings were cultivated with distilled water and 6-benzyl adenine(8 mg / L),respectively,and the proteins expression profile of functional leaves were investigated by using the approach of proteomics. Proteins were extracted from rice functional leaf and separated by two-dimensional gel electrophoresis,then 22 differential expression proteins that induced by 6-benzyl adenine were obtained,among which 15 proteins were identified by matrix-assisted laser desorption / ionization-time of flight mass spectrometry,and categorized into 4 groups of functions including photosynthesis,respiration,hormone metabolism and stress response. Results showed that under nutrient stress condition,6-benzyl adenine had benifical effect on rice plant: the expression abundance of proteins associated with photosynthesis and respiration increased,and proteins associated with contabescence were down-regulated in expression abundance. Thereby,application of 6-benzyl adenine chould enhanced the photosynthesis and respiration directly, provided the material and energy for plant growth and defered rice contabescence.

Key words: Rice, 6-benzyl adenine, Contabescence, Proteomics

CLC Number:

S312.03
Q78

SHAO Cai-hong, TANG Xiu-ying, LI Ming-xin, LI Yao, WANG Ping, CHEN Jin, XIE Jin-shui. Proteomics Analysis of Mechanism of 6-benzyl Adenine Deferring Contabescence of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 14-19. doi: 10.7668/hbnxb.2014.01.003.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.01.003

http://www.hbnxb.net/EN/Y2014/V29/I1/14

References

1 吴岳轩，吴振球丁酸对杂交水稻根系代谢活性及叶片衰老进私I的影响[J]杂交水稻，1991(4);29-34
2 王少先，彭克勤，萧浪涛双氰氨对水稻根系及光合特性和经济性状的影响[J]湖南农业大学学报:自然科学版，2003,29(1);18-21
3 王丰，程方民植物激素与水稻产量的关系及其在生产上的应用[J]现代化农业, 2003 (10); 20-21
4 陶龙兴，王熹，黄效林，等植物生长调节剂在农业中的应用及发展趋势[J]浙江农业学报，2001,13(5);322-326
5 黄静静，王绍华，李刚华，等6-节基腺嘌吟对水稻颖花分化影响机制的研究[J]南京农业大学学报，2009,32(3):8-13
6 杨知建，徐庆国，朱春生，等6-BA处理对水稻根系中后期生长的影响[J]湖南农业大学学报，2009,35(5):462-465
7 蔡永萍，陈静娴，聂凡水稻抽穗期喷施6-BA, ABA对旗叶衰老及同化物运输的影响[J]安徽农业科学，1996,24(2):113-1158 杨安中，牟筱玲，李孟良，等喷施细胞分裂素类物质对地膜旱作水稻防衰及增产效应[J]水土保持学报，2008，19(2):199-200
9 郭彦，张文会，巍秀俭，等6-BA对干旱胁迫条件下水稻芽期生长的影响[J]安徽农业科学，2005,33 (9):1992-1993
10 梁建秋，梁颖几种生长物质对油菜幼苗抗湿性的影响[J]西南师范大学学报，2009,34(1);s8-6211 徐皓6-BA对蚕豆离体叶片衰老的延缓作用[J] 江苏农业科学，2008(4);49-51
12 Wang X C，Li X F,Li Y X. A modified coomassie bril-liant blue staining method at nanogram sensitivity com-patible with proteomic analysis[J]Biotechnology Let-ters,2007,29:1999-1603
13 夏其昌，曾嵘蛋自质化学与蛋自质组学[M]北京:科学出版社，2004: 278
14 谢金水，邵彩虹，唐秀英，等养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋自质组学分析[J]中国水稻科学，2011,28(2);143-149
15 Hajduch M，Rakwal R,Garawal G K,et al. High-resolu-tion two-dimensional elecarophoresis separation of pro-teins from metal-stressed rice (Oryza. native L)leaves: Drastic: reducaions/fragmentation of ribulose-1,s-bispho-tosphate carboxylase/oxygenase and inducaion of stress-related proteins[J]Elecarophoresis，2001，22(13): 2824-2831
16 瞿礼嘉植物生物化学与分子生物学[M]北京:科学出版社，2004 } 131-483
17 Wollman F A, Minai L, Nechushtai R. The biogenesis and assembly of photosynt hetic proteins in thylakoid membranesl[J]Biochim Biophys Acta, 1999，1411 (1);21-85
18 Friso G, Giacomelli L, Ytterberg A J, et al. In depth a-nalysis of the thylakoid membrane proteome of Arabidop-si,s thalia}aa chloroplasts new proteins, new funcaions,and a plastid proteome database[J]Plant Cell, 2004，16 (2);478-499
19 Lea P J, Millin B J. Alternative route for nitrogen in higher plants [J]. Nature,1974,251(5476); 614-616
20 Lothier J, Gaufichon L, Sormani R, et al. The cytosolic glutamine synthetase GLN1;2 plays a role in the control of plant b owth and ammonium homeostasis in Arabidop-si.s rosettes when nitrate supply is not limiting [J]. Jour-nal of Experimental Botany,2011,62(4):1375-1390
21 Hirel B，Benin P,Quillere I, et al. Towards a better un-derstanding of the genetic: and physiological basis for ni-trogen use elficiency in maize[J]Plant Physiology, 2001，125(3):1258-1270
22 Harrison J, Crescenzo M P, Sene 0, et al. Does lowering glutamine synthetase acaivity in nodules modify nitrogen metabolism and growth of Lotus. japouicu.s[J]PlantPhysiology,2003，133(1);253-262
23 Cren M, Hirel B. Glutamine synthetase in higher plants:regulation of gene and protein expression from the organ tothe ce11[J]. Plant Cell Physiolog,1999, 40(12):1187-1193
24 Jones R J,Schreiher B M N. Role and funcaion of cyto-kinin oxidases in plants[J]Plant Growth Regulation,1997,23(1-2):122-134
25 Werner T, Motyka V, Strnad M, et al. Regulation of plantgrowth by cytokinin [J]. Proceedings of the National A-cademy of Science of USA, 2001，98(18):10487-10492
26 王军，侯丙凯植物小分子化合物的糖基化与糖基转移酶[J]植物生理学通讯，2008, 44 (5); 997-1002
27 吴华，严定平，罗越华水稻磷酸醋酶2A基因蛋自的原核表达载体构建、表达和纯化[J]贵州科学，2009,27(2);45-49
28 龚宁萍，陈朝银耐热碱性磷酸醋酶的研究进展[J]药物生物技术，2004,11(3);207-210
29 Singla-Pareek S L,Reddy M K,Sopory S K. Genetic: en-gineering of the glyoxalase pathway in tohacco leads toenhanced salinity tolerance[J]. Proceedings of the Na-tional Academy of Science of USA, 2003，100(25):14672-14677
30 Collinge M，Boller T. Differential inducaion of two potatogenes，Stprx2 and StNAC，in response to infecaion byPhytophthora cafe.stana.s and to wounding[J]Plant Mo-lecular Biology,2001,46(5);521-529
31 Fujita M, Fujita Y, Maruyama K, et al. A dehydrationin-duced NAC protein, RD26，is involved in a novel ABA-dependent sue signaling pathway[J]Plant Journal,2004,39(6);863-876
32 Tran L S,Nakashima K,Sakuma Y,et al. Isolation andfuncaional analysis of Arabidop.si,s stress-inducible NACtranscription facaors that bind to a drought-responsivecis-element in the early responsive to dehydration stress1 promoter[J]The Plant Ce11,2004，16(9):2481-2498
33 Delessert C，Kazan K,Wikon IW，et al. The transcrip-tion facaor ATAF2 represses the expression of pathogene-sis-related genes in Arabidop.si,s[J].. Plant Journa1,2005，43(5);745-757
34 孙虎，薛保国，杨丽荣，等植物硫氧还蛋自系统[J]基因组学与应用生物学，2010, 29 (4); 748 -753
35 Kampranis S C，Damianova R, Atallah M, et al. A novelplant glutathione S-transferase/peroxidase suppressesBax lethality in yeast [J]. Journal of Biological Chemis-try,2000,275(38);29207-29216
36 王丽，王晓丽，刘仕，等植物草酸氧化酶及其基因的研究进展[J]中国农学通报，2010,26(7);48 -51

[1]	WANG Yanning, HUANG Tao, WU Guangliang, HUANG Shiying, ZHONG Qi, WANG Peng, YANG Mengmeng, LI Caijing, CHENG Qin, HE Haohua, JIN Wei, GUO Ling, BIAN Jianmin. Transcriptome Analysis of Rice Bud Stage in Response to Cadmium Stress [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 25-35.
[2]	ZHOU Xinqiao, CHEN Dagang, SHAN Zelin, GUO Jie, CHEN Pingli, CHEN Ke, LIU Chuanguang, CHEN Youding. Study on Dynamic Plant Type Characteristics of Different Double Cropping Super Rice in South China [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 68-77.
[3]	LI Xia, LUO Lihui, ZHOU Ya, YANG Dingqing, WANG Peng, LI Sen. Effects of Straw Returning on Soil Active Organic Carbon Components and Enzyme Activities in Rice-Rape Rotation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 124-131.
[4]	WANG Ya, WANG Yuetao, SHEN Guanwang, WANG Fuhua, WANG Shengxuan, BAI Tao, YIN Haiqing. Improvement of Rice Blast Resistance by Pyramiding the R Gene Pigm and the Non-R Gene bsr-d1 [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 157-165.
[5]	LI Wenxiu, DAI Li, WANG Lihuang, FANG Baohua, WANG Xuehua. Carbon and Nitrogen Metabolism and Yield Composition in Multi-panicle and Large-panicle Super Rice Varieties at Young Panicle Differentiation Stage [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 103-112.
[6]	SHI Ailong, WANG Lihuang, LI Wenxiu, WANG Xuehua. Effects of Water and Fertilizer on Agronomic Characters and Yield of Double-cropping Hybrid Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 145-157.
[7]	ZHOU Liping, ZHAO Qiu, ZHANG Xinjian, NING Xiaoguang, YUAN Liang, LI Yanting, ZHAO Bingqiang. Effect of New Type Synergistic Compound Fertilizer on Rice Nutrient Absorption and Yield [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 112-120.
[8]	LIANG Yugang, ZHOU Jing, YU Zhengjun, LI Jingyi, HUANG Huang, ZHAO Zhenghong. Effect of Rice-duck Farming on the Composition and Diversity of the Weed Community in Direct Seeding Rice Paddies [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 160-170.
[9]	HAN Zhenghong, DUAN Yuxuan, XU Shanbin, WANG Jingguo, LIU Hualong, YANG Luomiao, JIA Yan, XIN Wei, ZHENG Hongliang, ZOU Detang. *Improvement of Grain Shape in Rice by Knocking GS3* and GS9 via CRISPR/Cas9 System** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 9-17.
[10]	LI Fujian, XU Dongyi, LIU Kaili, ZHU Min, LI Chunyan, ZHU Xinkai, DING Jinfeng, GUO Wenshan. Effects of Tillage Methods on Growth and Physiology of Seedlings Stem and Tillers and Their Productivity of Wheat in Rice Stubble [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 58-67.
[11]	LIU Junfeng, LI Yimeng, LIANG Chao, ZHOU Chanchan, WANG Shu, JIA Baoyan, HUANG Yuancai, WANG Yan, WANG Yun. Effect of Nitrogen Application Pattern and Row Spacing on Canopy Structure and Yield of Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 77-85.
[12]	JING Lili, PENG Ting, ZHAO Yafan, ZHAO Shuaibing, WANG Tongtong, LI Yuan, CHENG Yuan, DU Yanxiu, ZHANG Jing, SUN Hongzheng, ZHAO Quanzhi. Regulatory Effects of Two Grain Filling Regulators on Inferior Spikelets Filling of Large Panicle Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 86-94.
[13]	XU Lingqi, GUO Xiaohong, ZHANG Jianing, ZHAO Yang, LI Xiaolei, LIU Shaofeng, CUI Zhiyuan, AN Yiliang, LÜ Yandong. The Effect of Organic Fertilizer on the Quality of Dry Direct-seeding Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 137-146.
[14]	GAO Suhong, GE Changhong, ZHOU Guona, YAO Shujuan, LU Changkuan, GAO Baojia. *Establishment and Optimization of Bidirectional Electrophoresis System of Total Protein from Cabernet sauvignon* Buds Induced by Apolygus lucorum Feeding** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 181-187.
[15]	CHEN Ziqi, WANG Weiping, ZHAO Hongqiang, WANG Hao, HAN Xiao, YANG Luomiao, XIN Wei, LIU Hualong, ZHENG Hongliang, WANG Jingguo. *QTL Analysis of Rice Cold Tolerance at Germination and Bud Stage Utilizing Indica-Japonica* RIL Population** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 50-57.

Please choose a citation manager

Content to export