Abstract: Using the transgenic gsh I gene Arabidopsis thaliana as materials,the contents of gsh and MDA,the electrolyte leakage(EL) and the activities of GR,SOD and CAT in the transgenic plants were measured to study the function of gsh I gene and analyze physiological mechanisms of transgenic Arabidopsis thaliana. The contents of gsh increased in both transgenic and control plants when the plants were subjected to drying treatment,however, the gsh production rate was higher in transgenic plants than that in control plants. gsh synthesis is induced in both transgenic and control plants with drougth stress,but the gsh I gene expression in the transgenic plants enhanced gsh synthesis level. The activity of GR in transgenic plants was higher than that in control plants. GR activity increases leads to a higher ratios of gsh to GSSG in transgenic plants and oxidative gsh is reduced. gsh regeneration system is launched with drougth stress. The leaves water content did not show obvious differences in changes between the transgenic and control plants with drougth stress. However,the MDA contents and electrolyte leakage(EL)(Conductivity) increased in both transgenic and control plants,but those of the transgenic plants was slower than those of control plants. The transgenic plants improved the stability of the membrane system(Antioxidation). The activities of SOD and CAT decreased in both transgenic and control plants,but did not show obvious differences. gsh is different from SOD and CAT in eliminating ROS.

Key words: gsh I gene, Arabadopsas thalaana, Transgene, Drought tolerance, Physiological and biochemical index

CLC Number: 

• Q78