The Identification of Drought Tolerance-related Transcription Factors in Early Developing Tassel of Maize

doi:10.7668/hbnxb.2018.04.001

Abstract

Abstract: To explore more drought-related transcription factors genes in early growth and development of tassels, two drought-tolerant (DT) Tie 7922, X178 and two sensitive maize inbred lines (DS) Ji 81162, Dan 340 were used. The potted planting method was used to cultivate the seedlings, and the materials were treated by artificial simulated field drought stress and normal irrigation under the same and suitable water conditions. RNA-Seq technique was used to detect the changes in transcriptional level of the gene in drought related transcription factor family in the early stage of maize ear development compared with the normal irrigation treatment. The results showed that 287 genes were differently expressed under drought stress, and 34 of them are related to drought tolerance regulation revealed by GO enrichment and annotation, including 6 commons in two drought-tolerant line, 3 commons in two sensitive line, and all with uniform expression pattern, and 1 detected in four inbreds. The results provided the basic knowledge for drought tolerant gene identifition and molecular breeding of maize.

Key words: Maize tassel, Transcription factors, Drought tolerance

CLC Number:

Q78
S513.03

WU Yongbo, HAO Zhuanfang, WANG Nan, SONG Jie, ZHOU Yueheng, LIU Bojuan, ZHU Hanyong, DI Hong, WANG Zhenhua, LI Xinhai. The Identification of Drought Tolerance-related Transcription Factors in Early Developing Tassel of Maize[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 1-8. doi: 10.7668/hbnxb.2018.04.001.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2018.04.001

http://www.hbnxb.net/EN/Y2018/V33/I4/1

References

[1] Wang X,Wang H,Liu S,et al. Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings[J]. Nature Genetics,2016,48(10):1233-1241.
[2] Yu C. China's water crisis needs more than words[J]. Nature,2011,470:307.
[3] Xiang Y,Sun X,Gao S,et al. Deletion of an endoplasmic reticulum stress response element in a ZmPP2C-A gene facilitates drought tolerance of maize seedlings[J]. Molecular Plant,2017,10(3):456-469.
[4] Ashraf M. Inducing drought tolerance in plants:recent advances[J]. Biotechnology Advances,2010,28(1):169-183.
[5] Bruce W B,Edmeades G O,Barker T C. Molecular and physiological approaches to maize improvement for drought tolerance[J]. Journal of Experimental Botany,2002,53(366):13-25.
[6] 李亮. 玉米雄穗响应干旱胁迫的主要代谢途径解析及耐旱SNAC基因筛选鉴别[D]. 乌鲁木齐:新疆农业大学,2015.
[7] Zhang X,Liu X,Zhang D,et al. Genome-wide identification of gene expression in contrasting maize inbred lines under field drought conditions reveals the significance of transcription factors in drought tolerance[J]. PLoS One,2017,12(7):e0179477.
[8] Gujjar R S,Akhtar M,Singh M. Transcription factors in abiotic stress tolerance[J]. Indian Journal of Plant Physiology,2014,19(4):306-316.
[9] Li L,Ma Y W,Zhang S H,et al. Zea mays NAC transcription factor family members:their genomic characteristics and relationship with drought stress[J]. Research Journal of Biotechnology,2015,10(11):63-77.
[10] Liu S X,Wang X L,Wang H W,et al. Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L.[J]. PLoS Genetics,2013,9(9):e1003790.
[11] Ma H L,Zhou H L,Zhang H Y,et al. Cloning and expression analysis of an AP2/ERF gene and its responses to phytohormones and abiotic stresses in rice[J]. Rice Science,2010,17(1):1-9.
[12] Wei K F,Chen J,Chen Y F,et al. Molecular phylogenetic and expression analysis of the complete WRKY transcription factor family in maize[J]. DNA Research:an International Journal for Rapid Publication of Reports on Genes and Genomes,2012,19(2):153-164.
[13] Mao H,Wang H,Liu S,et al. A transposable element in a NAC gene is associated with drought tolerance in maize seedlings[J]. Nature Communications,2015,6:8326.
[14] An X,Liao Y W,Zhang J Y,et al. Overexpression of rice NAC gene SNAC1 in ramie improves drought and salt tolerance[J]. Plant Growth Regulation,2015,76(2):211-223.
[15] Ying S,Zhang D F,Fu J,et al. Cloning and characterization of a maize bZIP transcription factor,ZmbZIP72,confers drought and salt tolerance in transgenic Arabidopsis[J]. Planta,2012,235(2):253-266.
[16] Cai R H,Zhao Y,Wang Y F,et al. Overexpression of a maize WRKY58 gene enhances drought and salt tolerance in transgenic rice[J]. Plant Cell,Tissue and Organ Culture,2014,119(3):565-577.
[17] Li H,Gao Y,Xu H,et al. ZmWRKY33,a WRKY maize transcription factor conferring enhanced salt stress tolerances in Arabidopsis[J]. Plant Growth Regulation,2013,70(3):207-216.
[18] Morimoto K,Mizoi J,Qin F,et al. Stabilization of arabidopsis DREB2A is required but not sufficient for the induction of target genes under conditions of stress[J]. PLoS One,2013,8(12):e80457.
[19] Zheng J,Zhao J,Tao Y,et al. Isolation and analysis of water stress induced genes in maize seedlings by subtractive PCR and cDNA macroarray[J]. Plant Molecular Biology,2004,55(6):807-823.
[20] Li H Y,Wang T Y,Shi Y S,et al. Isolation and characterization of induced genes under drought stress at the flowering stage in maize( Zea mays)[J]. DNA Sequence:the Journal of DNA Sequencing and Mapping,2007,18(6):445-460.
[21] Poroyko V,Spollen W G,Hejlek L G,et al. Comparing regional transcript profiles from maize primary roots under well-watered and low water potential conditions[J]. Journal of Experimental Botany,2007,58(2):279-289.
[22] Hayano-Kanashiro C,Calderón-Vázquez C,Ibarra-Laclette E,et al. Analysis of gene expression and physiological responses in three Mexican maize landraces under drought stress and recovery irrigation[J]. PLoS One,2009,4(10):e7531.
[23] Li Y,Sun C,Huang Z,et al. Mechanisms of progressive water deficit tolerance and growth recovery of Chinese maize foundation genotypes Huangzao 4 and Chang 7-2,which are proposed on the basis of comparison of physiological and transcriptomic responses[J]. Plant & Cell Physiology,2009,50(12):2092-2111.
[24] Zheng J,Fu J,Gou M,et al. Genome-wide transcriptome analysis of two maize inbred lines under drought stress[J]. Plant Molecular Biology,2010,72(4/5):407-421.
[25] Zhuang Y,Ren G,Yue G,et al. Effects of water-deficit stress on the transcriptomes of developing immature ear and tassel in maize[J]. Plant Cell Reports,2007,26(12):2137-2147.
[26] Dewey,Lee,Brian,et al. Monitoring the expression of maize genes in developing kernels under drought stress using Oligo-microarray[J]. Journal of Integrative Plant Biology,2010,52(12):1059-1074.
[27] Wang Y J,Lu W J,Zhao J,et al. Transcriptome dynamics of dominant maize dwarf dwarf11(D11)revealed by RNA-seq and co-expression analysis[J]. Plant Molecular Biology Reporter,2017,35(3):355-365.
[28] Zhao C,Waalwijk C,De Wit P J,et al. RNA-Seq analysis reveals new gene models and alternative splicing in the fungal pathogen Fusarium graminearum[J]. BMC Genomics,2013,14(1):21.
[29] Li P,Cao W,Fang H,et al. Transcriptomic profiling of the maize( Zea mays L.)leaf response to abiotic stresses at the seedling sta drought resistance across multiple environments in maize[J]. Breeding Science,2011,61(2):101-108.
[30] Hao Z F,Li X H,Su Z J,et al. A proposed selection criterion for drought resistance across multiple environments in maize[J]. Breeding Science,2011,61(2):101-108.
[31] Wang N,Li L,Gao W W,et al. Transcriptomes of early developing tassels under drought stress reveal differential expression of genes related to drought tolerance in maize[J]. Breeding Science,2011,61(2):101-108.
[32] Kakumanu A,Ambavaram M M,Klumas C A,et al. Effects of drought on gene expression in maize reproductive and leaf meristem tissue revealed by RNA-Seq[J]. Plant Physiology,2012,160(2):846-867.
[33] Opitz N,Marcon C,Paschold A,et al. Extensive tissue-specific transcriptomic plasticity in maize primary roots upon water deficit[J]. Journal of Experimental Botany,2016,67(4):1095-1107.
[34] Jia J,Fu J,Zheng J,et al. Annotation and expression profile analysis of 2073 full-length cDNAs from stress-induced maize( Zea mays L.)seedlings[J]. The Plant Journal:for Cell and Molecular Biology,2006,48(5):710-727.
[35] Qiao Z,Li C L,Zhang W. WRKY1 regulates stomatal movement in drought-stressed Arabidopsis thaliana[J]. Plant Molecular Biology,2016,91(1/2):53-65.
[36] Sun Y,Yu D. Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement[J]. Plant Cell Reports,2015,34(8):1295-1306.
[37] Oh J E,Kwon Y,Kim J H,et al. A dual role for MYB60 in stomatal regulation and root growth of Arabidopsis thaliana under drought stress[J]. Plant Molecular Biology,2011,77(1/2):91-103.
[38] Yang J,Isabel Ordiz M,Jaworski J G,et al. Induced accumulation of cuticular waxes enhances drought tolerance in Arabidopsis by changes in development of stomata[J]. Plant Physiology and Biochemistry,2011,49(12):1448-1455.
[39] Fu Q T,Yu D Q. Expression profiles of AtWRKY25,AtWRKY26 and AtWRKY33 under abiotic stresses[J]. Hereditas,2010,32(8):848-856.
[40] Li J,Besseau S,Törönen P,et al. Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis[J]. The New Phytologist,2013,200(2):457-472.
[41] Avramova Z. The jasmonic acid-signalling and abscisic acid-signalling pathways cross talk during one,but not repeated,dehydration stress:a non-specific ‘panicky’ or a meaningful response?[J]. Plant,Cell & Environment,2017,40(9):1704-1710.
[42] Thatcher S R,Danilevskaya O N,Meng X,et al. Genome-Wide analysis of alternative splicing during development and drought stress in maize[J]. Plant Physiology,2016,170(1):586-599.
[43] Wang Y,Wang Q,Liu M L,et al. Overexpression of a maize MYB48 gene confers drought tolerance in transgenic Arabidopsis plants[J]. Journal of Plant Biology,2017,60(6):612-621.
[44] Jeong J S,Kim Y S,Baek K H,et al. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions[J]. Plant Physiology,2010,153(1):185-197.

Please choose a citation manager

Content to export