The Temporal Spatial Expression of Gibberell in Key Metabolic Enzyme in Upland Cotton

doi:10.7668/hbnxb.2018.04.002

Abstract

Abstract: To reveal the relationship between the key enzyme gene expression of gibberellin synthesis pathway and different tissues or stages of growth, the CCRI49 cotton was selected as material, and relative quantification Real-time PCR was developed to determine the gibberellin key gene expression of different cotton organizations in different stages of development. The results showed that, in seedling, the expression level of all gibberellin synthesis pathway genes was higher in stem, except GA2ox1 gene. In full-blooming stage, the expression of GA2ox1, GA3ox1, GID1B in root, CPS1, GA20ox1, GA3ox1, GID1B in stem and GA3ox1 in leaf had an up-regulation. In boll opening stage, the expression levels of GA2ox1 and GA3ox1 were up-regulated in root, while the remaining genes were down-regulated. The expression levels of KS and GID1B in stem were down-regulated, while the other genes were up-regulated, with GA2ox1 rise in maximum. And GA2ox1 in the leaf was down-regulated and the rest were up-regulated. In full-blooming stage, the high expression of GA20ox1 gene provided the necessary active gibberellin level for the elongation and growth of the plant stem, and the GA3ox1 gene might be associated with flowering and bell. In boll opening stage, the expression of GA2ox1 gene in root and stem gradually increased to reduce the synthesis of active, gibberellic acid. The gene expression levels of GA3ox1 and GA20ox1 in leaf tissues were raised to provide the necessary hormone levels for cotton boll growth and dehydration. The results indicated that cotton plant by changing the key enzymes gene expression of gibberellin synthesis metabolic pathway to regulate plant growth and development, and the purpose gene expression volume trends exist a certain difference along with the growth and development. It provides a theoretical basis for studying the regulation mechanism of gibberellin on upland cotton, which can help accelerate the improvement of variety and germplasm innovation.

Key words: Cotton, Gibberellin, Gene expression, Different tissues, Growth and development

CLC Number:

Q786

SHI Jianbin, WANG Ning, ZHOU Hong, XU Qinghua, QIAO Wenqing, YAN Gentu. The Temporal Spatial Expression of Gibberell in Key Metabolic Enzyme in Upland Cotton[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 9-16. doi: 10.7668/hbnxb.2018.04.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2018.04.002

http://www.hbnxb.net/EN/Y2018/V33/I4/9

References

[1] 李四游. 赤霉素对莲株型影响及其相关基因克隆与时空表达分析[D]. 武汉:华中农业大学,2015.
[2] 石建斌. 马铃薯赤霉素合成代谢途径关键酶基因GA2ox1, GA20ox1的克隆与功能分析[D]. 西宁:青海大学,2016.
[3] 虞慧芳,曹家树,王永勤. 植物矮化突变体的激素调控[J]. 生命科学,2002,14(2):85-88,76.
[4] 王海波,邹竹荣,龚明. 小桐子赤霉素20氧化酶的基因克隆及低温下表达分析[J]. 生物技术通报,2015,31(10):140-148.
[5] 孙同玉,祝娟,孙鹏,等. 西洋参赤霉素20-氧化酶基因的克隆与序列分析[J]. 中草药,2015,46(11):1656-1660.
[6] 王增辉. 黄瓜赤霉素氧化酶基因GA20ox、Ga2ox、Ga3ox 的生物信息学及GA20ox1功能分析[D]. 泰安:山东农业大学,2014.
[7] 岳川,曾建明,曹红利,等. 茶树赤霉素受体基因CsGID1a 的克隆与表达分析[J]. 作物学报,2013,39(4):599-608.
[8] 陈敏,马琳,贾聪俊,等. 紫花苜蓿赤霉素受体基因的克隆及表达分析[J]. 西北植物学报,2016,36(11):2159-2166.
[9] 刘斌. 黄瓜赤霉素受体基因CsGID1a的功能验证与表达谱分析[D]. 北京:中国农业大学,2017.
[10] 李强,吴建明,梁和,等. 高等植物赤霉素生物合成及其信号转导途径[J]. 生物技术通报,2014(10):16-22.
[11] 鲁井云,卢龙,邢佳毅,等. 外施赤霉素对峰后葡萄坐果及VvAG 基因表达的影响[J]. 中国农业大学学报,2015,20(1):53-59.
[12] 王西成,任国慧,房经贵,等. 葡萄赤霉素合成相关基因克隆、亚细胞定位和表达分析[J]. 中国农业科学,2012,45(11):2224-2231.
[13] 王西成,吴伟民,房经贵,等. 葡萄赤霉素受体基因VvGID1A 的分离、亚细胞定位及表达分析[J]. 园艺学报,2013,40(5):839-848.
[14] 钟希琼,王惠珍. 高等植物赤霉素生物合成及其调节研究进展[J]. 植物学通报,2001,18(3):303-307.
[15] 王丽. 赤霉素合成抑制剂DPC调控棉花营养生长的分子机制研究[D]. 北京:中国农业大学,2014.
[16] 张国华,张艳洁,丛日晨,等. 赤霉素作用机制研究进展[J]. 西北植物学报,2009,29(2):412-419.
[17] 石建斌,杨永智,王舰. 马铃薯突变体赤霉素代谢关键酶基因差异表达分析[J]. 生物技术通报,2016,32(1):124-130.
[18] Prisic S,Peters R J. Synergistic substrate inhibition of ent-copalyl diphosphate synthase:a potential feed-forward inhibition mechanism limiting gibberellin metabolism[J]. Plant Physiology,2007,144(1):445-454.
[19] 柯贞进. 谷子萌发过程中贮藏物质变化及赤霉素代谢关键酶基因表达的分析[D]. 太谷:山西农业大学,2015.
[20] 李晨晨,侯雷,尹亮,等. 水稻极矮突变体s2-47对赤霉素的响应及基因定位研究[J]. 作物学报,2013,39(10):1766-1774.
[21] Itoh H,Tatsumi T,Sakamoto T,et al. A rice semi-dwarf gene,Tan-Ginbozu(D35),encodes the gibberellin biosynthesis enzyme,ent-kaurene oxidase[J]. Plant Molecular Biology,2004,54(4):533-547.
[22] 郑伶杰,马宏,张媛,等. 苹果矮化砧木GA20氧化酶基因的克隆及其表达分析[J]. 华北农学报,2017,32(1):53-59.
[23] 王成祥,李长生,侯蕾,等. 花生赤霉素2-氧化酶基因的克隆和表达研究[J]. 山东农业科学,2013,45(1):14-18,24.
[24] Spray C R,Kobayashi M,Suzuki Y,et al. The dwarf-1(dt)Mutant of Zea mays blocks three steps in the gibberellin-biosynthetic pathway[J]. Proceedings of the National Academy of Sciences of the United States of America,1996,93(19):10515-10518.
[25] Huang S,Raman A S,Ream J E,et al. Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis[J]. Plant Physiology,1998,118(3):773-781.
[26] Chiang H H,Hwang I,Goodman H M. Isolation of the Arabidopsis GA4 locus[J]. The Plant Cell,1995,7(2):195-201.
[27] Itoh H,Tanaka-Ueguchi M,Kawaide H,et al. The gene encoding tobacco gibberellin 3beta-hydroxylase is expressed at the site of GA action during stem elongation and flower organ development[J]. The Plant Journal,1999,20(1):15-24.
[28] Lange T R S. Expression of a gibberellin 2β. 3β-hydroxylase cDNA from pumpkin endosperm[J]. Plant Cell,1997,9(8):1459-1467.
[29] Lee D J,Zeevaart J A. Differential regulation of RNA levels of gibberellin dioxygenases by photoperiod in spinach[J]. Plant Physiology,2002,130(4):2085-2094.
[30] Yamaguchi S,Kamiya Y. Gibberellin biosynthesis:its regulation by endogenous and environmental signals[J]. Plant & Cell Physiology,2000,41(3):251-257.

Please choose a citation manager

Content to export