Cloning and Expression Analysis of Arginase Gene GhARG1 cDNA from Gossypium hirsutum L.

doi:10.7668/hbnxb.2018.04.003

Abstract

Abstract: It is of great significance to investigate the response of arginase gene to abiotic stress and its physiological function in cotton. Bioinformatics analysis showed that the ORF sequence of GhARG1 was obtained by In silico cloning and RT-PCR technique. Salt stress, PEG6000 stress and ABA, SA and MeJA treatment cotton seedlings, and GhARG1 was expressed in E. coli prokaryotic. The ORF sequence contained 1 023 bp, and encoded 340 amino acids with typical conserved domains of arginase. Quantitative RT-PCR analysis showed that the expression of GhARG1 in leaves was up-regulated under stress condition of NaCl, PEG6000, and signal molecule of ABA, SA, respectively, but down-regulated by MeJA. The coding sequence of GhARG1 was fused to prokaroytic expression vector pCold-TF, and the combinant vectors were induced by IPTG and detected by SDS-PAGE. The molecule weight of protein GhARG1 was detected to be the expected 37 ku. The activity of arginase in the recombinant strain was 6 times that of the control. The results suggested that GhARG1, which coding product has arginase activity, might be involved in the response to salinity and drought stress through ABA and SA signaling pathway in cotton, and could lay foundation for further physiological functional identification.

Key words: Gossypium hirsutum L., Arginase, Abiotic stress, Signal molecule, Functional identification

CLC Number:

Q78
S562.03

WANG Huifei, FENG Xue, ZHANG Yiming, FENG Lifeng, ZHANG Yu, CHEN Guang, SUN Yanxiang. Cloning and Expression Analysis of Arginase Gene GhARG1 cDNA from Gossypium hirsutum L.[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 17-24. doi: 10.7668/hbnxb.2018.04.003.

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