Genome Wide Identification and Expression Analysis of AMT Gene Family Members in Brassica napus

doi:10.7668/hbnxb.20192007

Abstract

Abstract: Understanding the roles of BnaAMTs in allotetraploid Brassica napus could provide a theoretical basis for further cloning and using these genes to improve nitrogen use efficiency and ammonium toxicity resistance in Brassica napus. 20 BnaAMTs genes were identified by bioinformatics. Among them, 14 were AMT1 subfamily members and 6 were AMT2 subfamily members. They were unevenly distributed on the 12 chromosomes of Brassica napus. The results of conserved motif analysis showed that the protein sequences of BnaAMTs subfamilies were highly conserved, but the differences between subfamilies were large. The expression profile of BnaAMTs gene in different tissues of rapeseed indicated that the expression of this gene family was tissue-specific. The results of transcriptome sequencing showed that in root, BnaA7. AMT1; 3 had the highest expression level when the roots were fed high concentrations of ammonium and nitrate. Under the condition of nitrogen deficiency, the expression of BnaA5. AMT1; 1 was the highest in root. Under the above treatments, BnaC6. AMT1; 1 had the highest expression in shoot. Under the condition of boron deficiency or phosphorus deficiency, the overall expression of BnaAMTs gene in rapeseeds showed an upward trend. However, under salt stress, the expression level decreased. Cadmium stress had little effect on the expression of BnaAMTs. These genes may involve in the interaction of ammonium with other essential nutrients and play a certain role in Cd and salt stress. This study lays a foundation for further analysis of the molecular function of AMT family genes in Brassica napus.

Key words: Brassica napus, AMT, Bioinformatic analysis, Abiotic stress, Transcriptional response

CLC Number:

S565.4

ZHOU Ting, YUE Caipeng, HUANG Jinyong, HUA Yingpeng. Genome Wide Identification and Expression Analysis of AMT Gene Family Members in Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 10-22. doi: 10.7668/hbnxb.20192007.

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