Heterologous Overexpression of Atvip1 Gene Enhances the Resistance of Transgenic Sorghum to Saline-alkali Stress

doi:10.7668/hbnxb.20191908

Abstract

Abstract: In order to study the tolerance of heterologously overexpressed Atvip1 gene in sorghum to defense saline-alkali stress and the corresponding growth, NaHCO₃:Na₂CO₃ of 5:1 solution with 75 mmol/L and pH 9.63 was used in sorghum at the stage of three leaves and one heart. The root growth index, chlorophyll content, antioxidant enzyme activity and MDA content were measured at 0, 4, 12, 24, 72, and 120 h of stress. The results indicated that the heterologous overexpression of Atvip1 gene could alleviate the damage of saline-alkali stress on the growth of sorghum seedlings, increase the root surface area and root volume, the number of root tips and branches, and also cause the browning of sorghum main roots to appear later and mild symphonys, and the earlier and more lateral roots occurrence. The new leaves could still be normally extended at 72 h and present little effect on the growth of aboveground. Overexpression of Atvip1 gene could increase the activity of O₂^-· resistance, decrease the content of MDA and enhance the activities of antioxidant enzymes in transgenic sorghum roots. SOD, CAT and GR had obvious effects at 4-12 h during the early stage of stress, respectively. All enzymes played roles during the middle of stress at 24-72 h. CAT and GSH-PX played important roles at the later stage of stress at 120 h. On the base of differential transcriptome analysis of saline-alkali stress, COG analysis of differentially expressed genes(DEGs) showed that defense mechanisms accounted for a relatively large proportion during various periods, and 42 DEGs related to antioxidant enzymes were obtained. Heterologous overexpression of Atvip1 gene can improve the resistance of transgenic sorghum to saline-alkali stress by alleviating the effects on photosynthesis, growth and development, reducing the damages of reactive oxygen species and membrane damage.

Key words: Sorghum, Atvip1, Overexpression, Saline-alkali stress, Growth and development, Resistance

CLC Number:

Q78
S514.03

CHENG Xinran, CAI Xinyue, YAN Wenxiang, NIU Jiangshuai, WU Rong, NIU Tingli, MU Yunjing, DAI Lingyan. Heterologous Overexpression of Atvip1 Gene Enhances the Resistance of Transgenic Sorghum to Saline-alkali Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 1-9. doi: 10.7668/hbnxb.20191908.

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