Cloning and Expression Analysis of Transcription Factor Gene GhERFB 101 Related to Resistant Fusarium oxysporum f.sp. vasinfectum in Cotton

doi:10.7668/hbnxb.2015.01.011

Abstract

Abstract: In order to explore the relationship between ERF transcription factor family and cotton resistance to Fusarium wilt, and provide new gene resources for breeding work of sea island cotton resistance to Fusarium wilt.Before this study, we constructed a digital expression profiling of cotton root tissues infected by Fusarium oxysporum f.sp. vasinfectum (Fov)in different time, then we found a query sequence in it, through the method of silico cloning and RT-PCR, we cloned a new ERF-B1 subgroup transcription factor gene, named GhERFB101 (GenBank:KF850521)from the roots of Zhongmiansuo 12 which was a high resistant cultivar to the infection of Fov., sequence analysis indicated that the ORF of GhERFB101 was 738 bp encoding 245 amino acids and a conserved AP2/ERF domain, and closed to Arabidopsis AtERF11 in the phylogenetic relationship.Real-time RT-PCR analysis indicated that, the relative expression of GhERFB101 decreased in resistant cultivar after the treatment of Fov.while in the susceptible cultivar the gene expression was up regulation.With the treatment time going on, the expression level increased first and decreased then.It reached the highest after 12 h and the lowest after 48 h.After the treatment of ethylene and jasmonic acid, the expression also showed the same trend like before, the expression level reached the maximum at 2 h after the treatment of ethylene, and it reached the maximum at 1 h after the treatment of jasmonic acid.While the changes of salicylic acid treatment was not significant;we speculated that GhERFB101 may involve in jasmonic acid and ethylene signal pathway to defense Fov.

Key words: Cotton, ERF-B1 transcription factors, Fusarium oxysporum f.sp. vasinfectum, Gene clone, Expression analysis

CLC Number:

Q78
S562.03

HAN Ze-gang, ZHAO Zeng-qiang, HE Lan-lan, CHAI Meng-liang, LI Hui-hui, ZHANG Wei. Cloning and Expression Analysis of Transcription Factor Gene GhERFB 101 Related to Resistant Fusarium oxysporum f.sp. vasinfectum in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 66-72. doi: 10.7668/hbnxb.2015.01.011.

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