The Construction of a Vector for Endosperm-specific Expression of IPT Gene and It's Transformation in Rice

doi:10.7668/hbnxb.2015.01.010

Abstract

Abstract: In order to specifically express IPT gene in the endosperm of developing rice seed, the binary vector p1300- pPG-5α-IPT-Nos was constructed, firstly, the promoter of PG-5α was obtained by PCR amplification from rice genomic DNA(variety:9311)and p1300- pPG-5α was constructed by inserting pPG-5α into p1300, then the IPT-Nos fragment was obtained by cutting pSG516 with Nco Ⅰ and Spe Ⅰ.After IPT-Nos inserted into p1300- pPG-5α, p1300- pPG-5α-IPT-Nos was constructed. pPG-5α-IPT-Nos was transformated into Oryza sativa (subsp.japonicacv.Nipponbare) cells by agrobacterium mediated method and regenerative seedlings were obtained.The results of PCR showed that about 81.3% seedlings were positive.

Key words: The promoter of PG-5α, IPT, p1300- pPG-5α-IPT-Nos, Oryza sativa, Transformation

CLC Number:

ZHANG Hong-xin, WANG Gui-lan, CHEN Chao, ZHAO Pu, MA Chun-hong. The Construction of a Vector for Endosperm-specific Expression of IPT Gene and It's Transformation in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 61-65. doi: 10.7668/hbnxb.2015.01.010.

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http://www.hbnxb.net/EN/Y2015/V30/I1/61

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