Abstract: A Receptor-like kinase(RLK)gene, OsSIK1,plays important roles in drought stress-tolerance in rice,through the activation of the antioxidative system.To make maize plants have much more drought resistance genes and further to obtain drought tolerance maize germplasm.In this study, OsSIK1 gene of rice was transformated into maize inbred Zheng 58 plants by pollen-mediated method.First,transgenic plants in T₁,T₂ and T₃ were detected by Kanamycin resistance screening,PCR and Southern Blotting,transgenic plants were obtained from T₁ and pure transgenic lines was obtained from T₃.Next,drought resistance analysis to transgenic maize plants and non-transformation control plants were conducted under the condition of 16.1% PEG drought stress.The results showed that compared with non-transgenic plants,the seedling leaf relative water content,chlorophyll content and SOD activity of transgenic plants were increased by 7.4%-19.8%,11.3%-106.9% and 45.8%-93.4%,respectively;furthermore,the relative conductivity and MDA content were decreased by 35.4%-58.1%,and 25.7%-50.4%,respectively.All the physiological indexes under the drought stress proved that transgenic OsSIK1 gene improved the drought resistance of transgenic maize plants,further analysis found that there were significant differences on drought tolerance between 5 transformed lines and their control groups,and their performance of field were superior to that of non-transgenic maize seedlings.At last,5 transgenic maize inbred lines were obtained,which suggested that genetically modified maize has improved the drought resistance by introducing foreign OsSIK1 gene of rice.

Key words: OsSIK1 gene, Pollen mediated transformation, Transgenic maize, Drought resistance

CLC Number: 

• S511