Abstract: To provide one elite drought-resistance gene resource for maize breeding program,a trehalose-6-phosphate synthase(TPS) gene sequence was obtained from Gossypium arboretum by bioinformatics analysis and molecular experimental operation. The gene was optimized and synthesized based on maize preferred codons. Afterwards,the gene was cloned into expression plasmids by multiple DNA restriction enzyme digestion and molecular ligation operations,and further transformed into E.coli and yeast cells to identify its expression status. Finally,the new TPS gene was cloned into plant expression vector. The results showed that the new synthesized gene had 2 586 bp in length,it contained a whole open reading frame and encoded 861 amino acids;constructed prokaryotic and eukaryotic expression plasmids can agree with our predicted results,this suggested that the gene could be used for cell transformation experiment;the synthesized TPS gene could effectively express trehalose-6-phosphate synthase in E.coli and yeast cells;the TPS gene was successfully ligated into plant expression vector pCAMBIA-2300,and further transformed into engineering strain LBA4404 of Agrobacterium. Comprehensively,it was concluded that a new TPS gene was obtained in this experiment,which could express trehalose-6-phosphate synthase in prokaryotic and eukaryotic cells. Therefore,the constructed plant expression plasmid can be directly applied in maize drought-resistance breeding program.

Key words: Maize, Trehalose-6-phosphate synthase, Gene, Drought resistance

CLC Number: 

• S513.03

• Q78