Abstract: To identify the miRNAs and their response regular patterns to salt stress and drought stress,rice seedlings at three leaves stage were used to quantify the expression of miRNAs and their targets when treated with salt and drought stresses at 0,3,6,12,24,48 h by qRT-PCR. The results demonstrated that expression patterns of miRNAs were affected by space-time and tissues under salt and drought treatments. Further analysis indicated that the expressions of miR156,miR164,miR167,miR169, and miR171 upregulated in roots with time and expressions of miR159,miR160,miR319,miR398, and miR1848 downregulated at 3 h and then upregulated when treated with salt(NaCl). And the 10 miRNAs were performed their lowest expressions in shoots at 3 or 6 h under NaCl treatment. On the other hand,the expressions of the most of the 10 miRNAs downregulated in roots generally and expressions of miR156,miR159 and miR160 downregulated in shoots and the expression patterns of miR167,miR169,miR319,miR398, and miR1848 down regulated first and then upregulated when treated with drought(PEG). Furthermore,expression patterns of targets were also affected by space-time and tissues. And only few expression patterns of the 10 miRNAs negatively correlated with its target,which implied the complexity of the regulation network of miRNA and its target in response to stresses.

Key words: Rice, Salt stress, Drought stress, miRNA, Expression

CLC Number: 

• Q78

• S511.03