Detection and Practical Evaluation of Molecular Markers Associated with Drought Resistance Based on Introduced Wheat Germplasm Resources

doi:10.7668/hbnxb.20191845

Abstract

Abstract: In order to effectively utilize the introduced wheat germplasm resources and evaluate the effectiveness of molecular markers associated with wheat drought resistance, 48 wheat germplasms were used to identify the relative germination rate of seeds under drought conditions, and 4 drought resistance related molecular markers were used to detect the distribution of drought resistance genes(1-feh-w3, Dreb-B1, NRX-B1 and FerA1). The results showed that the average relative germination rate of the introduced germplasms was 70.0%, the variation range was 34.9%-94.5%, and 85.4% of germplasm materials displayed moderate or above drought resistance level. There were 8 haplotypes in 4 drought resistance-related genes in the introduced wheat germplasms. The difference in the average relative germination rate between the 2 haplotypes of the Dreb-B1 gene and FerA1 gene reached significant levels, while there was no significant difference in the average relative germination rate between the Westonia type haplotype and Kauz type haplotype of the 1-feh-w3 gene, and the TaNRX-B1a haplotype and the TaNRX-B1b haplotype of the NRX-B1 gene;The haplotypes TaDreb-B1a, TaFer-A1 (H) were significantly positively correlated with the average relative germination rate, while 1-feh-w3 and NRX-B1 genes were not significantly correlated with the average relative germination rate. The average relative germination rate was the highest in the haplotype combination Westonia type/TaDreb-B1a/TaFer-A1 (H)/TaNRX-B1a, reaching 77.35%. The effectiveness of the molecular marker of the drought resistance gene Dreb-B1 was verified, and the two haplotypes of the 1-feh-w3 gene were invalid for drought resistance selection.

Key words: Wheat, Introduced germplasm, Drought resistance, Relative germination rate, Molecular marker

CLC Number:

S512.03

WU Xiaojun, HU Xigui, CHEN Xiangdong, JIANG Xiaoling, ZHANG Xuening, WANG Yuquan, DONG Na, HU Tiezhu, LI Xiaohui, RU Zhengang. Detection and Practical Evaluation of Molecular Markers Associated with Drought Resistance Based on Introduced Wheat Germplasm Resources[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 74-82. doi: 10.7668/hbnxb.20191845.

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