Drought Resistance Analysis of Ovate Family Proteins Transcription Factor AtOFP8

doi:10.7668/hbnxb.20190669

Abstract

Abstract: To explore the function of the Arabidopsis thaliana transcription factor AtOFP8 (At5g19650) and to clarify its mechanism of action, the Col wild type Arabidopsis thaliana, Atofp8-1 mutant and 35S:HA-AtOFP8 transgenic plants were used as test materials, method of simulating drought in nature using potted plants, continuous water control 15 d, through the phenotypic observation and analysis of the experimental materials, the relevant physiological indexes of the experimental materials under drought stress and the responses of the drought response genes in the plants were simultaneously determined. Results showed:with the increase of drought stress, 35S:HA-AtOFP8 overexpression had the highest seed germination rate and green leaf rate, while Atofp8-1 mutant had the lowest; When drought stress was 15 days, the content of MDA in the leaves of Atofp8-1 mutant, Col wild type and 35S:HA-AtOFP8 overexpression was increased by 229.8%, 130.4% and 71.1%, respectively. As the drought stress time increases, the activities of antioxidant enzymes, proline and soluble protein in leaves of Col, Atofp8-1 deletion mutant and 35S:HA-AtOFP8 overexpressing plants increased to varying degrees, however, the antioxidant enzyme activity, proline and soluble protein content in leaves of 35S:HA-AtOFP8 overexpression were consistently higher than Col wild type and Atofp8-1 mutant. Analysis of Real-time PCR showed that the expression of drought response genes (AtADH1,AtRD26,AtRDUF2,AtERD7) in 35S:HA-AtOFP8 overexpression was higher than that of Atofp8-1 mutant. This indicates that Arabidopsis thaliana transcription factor AtOFP8 confers higher drought tolerance.

Key words: Ovate family proteins, Transcription factor, AtOFP8, Drought resistance

CLC Number:

LIU Shouyin, TANG Yao, HAN Jialiang, CHANG Ying. Drought Resistance Analysis of Ovate Family Proteins Transcription Factor AtOFP8[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 98-106. doi: 10.7668/hbnxb.20190669.

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