Expression Pattern of ERD Genes in Maize and Drought Resistance Analysis of ZmERD6 Gene

doi:10.7668/hbnxb.20190812

Abstract

Abstract: The early responsive to dehydration(ERD)gene is a kind of functional gene that can respond quickly when plants encounter water stress,and plays an important role in slowing the stress of plants suffering from water stress. This type of gene is excavated and new drought-resistant varieties can be cultivated through molecular-assisted breeding. Therefore,based on previous studies,in order to further understand the ZmERD genes,this study screened five ZmERD genes based on drought stress transcriptome data. The proteins encoded by these genes were analyzed with AtERD protein,and the proteins encoded by the GRMZM2G181206 and GRMZM2G128641 genes were found to have higher homology with the proteins encoded by AT4G02900 and the AT1G32090 protein,respectively. The spatiotemporal expression analysis revealed that the GRMZM2G109201,GRMZM2G181206 and GRMZM2G12864 genes were constitutively expressed,and the GRMZM2G134192 gene was a specific expression,which was significantly highly expressed only in anthers. The GRMZM2G109201 gene belonging to the ERD6 subfamily was expressed in both drought-sensitive(B73)and drought-resistant(Zheng 58)inbred lines,and the expression level in Zheng 58 was significantly higher than that in B73. With the prolongation of drought stress,the expression level of GRMZM2G109201 gene was not significantly different in B73, but the expression level in Zheng 58 increased significantly. Subcellular localization revealed that the ZmERD6 protein encoded by the GRMZM2G109201 gene was located in the plasma membrane. We speculated that the GRMZM2G109201 gene was involved in drought stress and was positively induced.

Key words: Maize, ERD gene, Evolution, Expression pattern, Drought resistance, Subcellular localization

CLC Number:

S513

LIU Songtao, CAO Wenmei, ZHENG Beibei, ZHAO Wei. Expression Pattern of ERD Genes in Maize and Drought Resistance Analysis of ZmERD6 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 51-56. doi: 10.7668/hbnxb.20190812.

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