Abstract: In order to study the influence of silicon on the expression of pepper CaMADS-box gene under abiotic stress,such as the high temperature and salt stress,we used pepper 101 as experimental materials,the physical and chemical properties of encoding protein was analyzed,phylogenetic tree was constructed,subcellular localization was predicted through bioinformatics software on the base of the cloning of pepper CaMADS-box gene.The results showed that the cloned gene encoding protein CaMADS-box was hydrophilic protein,containing MADS domain structure,belonged to MADS gene families.And its subcellular localization was in the nucleus,the molecular evolutionary tree showed that close to Nicotiana,the similarity was 67%.Fluorescence quantitative analysis showed that CaMADS-box gene expression after high temperature stress and salt stress were presented first rise after the fall of the model, the difference was to peak at 48 h, under high temperature stress, salt stress peak at 24 h, silicon handle could induce gene expression CaMADS-box, under high temperature stress and salt stress were expressed at 12 h to reach peak, which suggested that CaMADS-box was a silicon quick response genes, speculated that the silicon handle in alleviating pepper abiotic stress such as high temperature and salt stress plays an important role.

Key words: Silicon, Capsicum annuum L., CaMADS-box gene, Abiotic stress, Expression analysis

CLC Number: 

• Q78