Acquirement of the Transgenic Maize Harboring Sb401 Gene

doi:10.3969/j.issn.1000-7091.2013.04.012

Abstract

Abstract: The purpose of this study was to improve the lysine content in corn，Sb401 gene was introdued into imma- ture embryo callus using particle bombardment．Regeneration into plants by selection for 1．5，3．0 mol/L bialaphos resist- ance. PCR detection in the obtained by plants and protein dipsticks testing．The results show that 38 transgenic plants were identified by PCR from 58 regenerated transformed plants obtained by selection for bialaphos resistance，31 transgen- ic plants was identified by test trip．It turned out that 31 transgenic plants has introduced target gene．

Key words: Maize, Transgene, Sb401, High lysine gene

CLC Number:

TIE Shuang-gui, CHEN Xiao-jie, YUE Run-qing, QI Jian-shuang, YAN Shu-feng, HAN Xiao-hua, XU Yu-ge,LIN Hong. Acquirement of the Transgenic Maize Harboring Sb401 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 66-68. doi: 10.3969/j.issn.1000-7091.2013.04.012.

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