The Molecular Characterization and Response Property to External Phosphorus of TaPT2; 1,a Phosphate Transporter Gene in Wheat

doi:10.7668/hbnxb.2014.01.001

Abstract

Abstract: Using a wheat phosphate transporter gene TaPT2; 1 that has not been characterized and functionally analyzed as the basis,the molecular characterization and response property of this gene to external Pi were studied in this study. The cDNA length of TaPT2; 1 is 2 094 bp,encoding a 568-aa polypeptide which harbors thirteen conserved membrane-spanning domains. After translation and sorted,TaPT2; 1 targets to the subcellular location of mitochondria. The expression of TaPT2; 1 exhibited a pattern to be strong under sufficient-Pi condition,and to be dramatically down-regulated in leaves and roots when subjected to low-Pi stress. In addition,the expression levels of TaPT2; 1 in leaves were significantly higher than roots under conditions of sufficient and low-Pi. The T 2 transgenic tobacco plants genetically transforming TaPT2; 1 promoter and the reporter gene GUS confirmed the results that obtained in wheat mentioned previously. The expression of GUS under the control of TaPT2; 1 promoter also showed to be higher under sufficient-Pi condition than under low-Pi condition,as well as to be stronger in leaves than in roots under both conditions of sufficient-and low-Pi. The Pi-responsive cis-acting regulatory elements,PIBS and PHO1 that are located at the promoter region of TaPT2; 1,are possibly involved in regulating this wheat phosphate transporter gene to express with a pattern of leaf predominant and induction by Pi sufficience. Furthermore,the downstream gene expression directed by TaPT2; 1 under sufficient-Pi condition displayed a typical circadian rhythm pattern of photo phase-high and dark phase-low and exhibited a obvious pattern of gradually up-regulated along with the growth progression. Our results in this study suggested that TaPT2; 1 play an important role in mediating the trans-portation of Pi from the cellular cytoplasm to the mitochondria.

Key words: Wheat, Phosphate transporter gene, Phosphorus level, Expression, Reporter gene

CLC Number:

CHEN Hong-mei, LI Xiao-juan, MA Chun-ying, DUAN Wei-wei, XIAO Kai. The Molecular Characterization and Response Property to External Phosphorus of TaPT2; 1,a Phosphate Transporter Gene in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 1-6. doi: 10.7668/hbnxb.2014.01.001.

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