Abstract: By CaMV35S promoter and Nos as the terminator,the overexpression vector of GhWRKY44 gene and verified the function of GhWRKY44 (Accession number:KJ801807) gene in tobacco ectopic expression were constucted.Overexpression vector was introduced into Agrobacterium GV3101 by electroporation method,positive bacteria strain was identified by screening PCR and positive colony was transferred into tobacco by Agrobacterium-mediated.After T₀ of transformed plants are detected by kanamycin,PCR detection,5 strains were detected by RT-PCR technique randomly selected from T₀ of transformed plants and 5 strains were positive.The results showed that not only GhWRKY44 gene was transferred into the tobacco genome but also the normal transcripted.T₁ lines of transgenic tobacco were analysed by using Real-time quantitative PCR (qRT-PCR) technique.The result showed that the wild type and transgenic GhWRKY44 tobacco were not inoculated with Fusarium oxysporum,the expression of PR5 and NPR1 expression obviously increased in transgenic GhWRKY44 plant.Wild type and transgenic plant after were infected by Fusarium oxysporum,the expression of PR1a, PR2 and NPR1 significantly increased in transgenic lines,but the expression of PR5 was higher in wild type than transgenic lines.The gene could be expressed in the tobacco and could activate parts of PRs'expression.But the function of GhWRKY44 still needs further exploration.

Key words: Tobacco, GhWRKY44, Genetic transformation, Pathogenesis-related gene

CLC Number: 

• S572.03