Transcriptome Analysis of the Effect of Different Rootstocks on the Resistance of Grafted Flue-cured Tobacco to Bacterial Wilt

doi:10.7668/hbnxb.20190870

Abstract

Abstract: In order to study the effect of different rootstocks on the disease resistance of tobacco grafted seedlings, and to clarify the mechanism of Yunyan 87 (Y87) as rootstock to improve the resistance of scion Hongdajinyuan (HD) to bacterial wilt, this study used split grafting and raw material band winding method to construct HD/HD grafted and HD/Y87 flue-cured tobacco seedlings respectively, using HD as the scion and Y87 as the rootstock. The PAL activity of HD/HD and HD/Y87 protective enzymes was measured. Finally, mRNA of 5 HD/HD or HD/Y87 were extracted and reverse transcribed to construct a total cDNA library by high-throughput sequencing technology and Illumina HiSeqTM 4000 sequencing platform. After sequencing and filtering low-quality Reads, the reference genome was compared with TopHat2, and the genes expression amount and differentially expressed genes were obtained by Cufflinks and DESeq software Heterogene expression. The results showed that the PAL enzyme activity of HD/Y87 was significantly higher than that of HD/HD at different sampling times, 48 414 474-48 697 874 Clean Reads and 38 272-40 938 genes were obtained from four samples, respectively, and the number of selective shear events of HD/Y87 was higher than HD/HD. After comparing the gene expression between HD/Y87 and HD/HD, 3 904 differential genes were obtained, 3 096 genes were up-regulated, 808 genes were down regulated; among the up-regulated genes, there were 3 genes ecoding phenylalanine ammonia lyases, 5 genes ecoding Myb family transcription factors, and 5 genes ecoding polyphenol oxidase involved in lignin synthesis, it also had 2 disease-related proteins and 3 genes ecoding ERF transcription factors. These results showed that Y87 as rootstock could improve the activity of HD PAL and gene expression of various resistance genes (including PAL, PPO and disease related protein) in scion, so as to improve the resistance of HD to bacterial wilt and other diseases.

Key words: Tobacco, Grafting, Transcriptome, Phenylalanine ammonia lyase, Polyphenol oxidase, Disease-related proteins

CLC Number:

S604
Q78

XIE Bing, ZHAN Xiaoxu, LUO Yanhong, LIU Binxiang, KONG Fanlei, YUAN Jichao. Transcriptome Analysis of the Effect of Different Rootstocks on the Resistance of Grafted Flue-cured Tobacco to Bacterial Wilt[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 177-186. doi: 10.7668/hbnxb.20190870.

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