The Exploration of the Effect of N -3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato

doi:10.7668/hbnxb.20190995

Abstract

Abstract: In order to determine the bacterial quorum sensing (QS) signaling molecule, N -acyl-homoserine lactones (AHLs) induce resistance to fungal diseases in plants. Tomato was used as the test material, Botrytis cinerea was used as the pathogen indicator bacteria, and AHLs signal molecules with different side chain length and side chain modification were pretreated to inoculate the pathogen bacteria, and the long chain signal molecule N-3-oxo-tetradecanoyl was found. 3OC14-HSL had the best resistance to gray mold. Real-time quantitative PCR detection of disease resistance genes revealed that the signal molecule 3OC14-HSL pretreatment significantly induced up-regulation of the genes PI1 and PI2 in the tomato and jasmonic acid (JA) signaling pathway compared with the untreated control group; However, the NPR1 gene related to the salicylic acid signaling (SA) pathway was significantly down-regulated, but the PR1 gene was not significantly changed. Further DAB staining, determination of hydrogen peroxide content, and detection of peroxidase (POD) and superoxide dismutase(SOD)enzyme activities revealed that 3OC14-HSL pretreatment caused a large amount of reactive oxygen species in tomatoes compared to the untreated control group. 3OC14-HSL also could maintain high POD, SOD activity. These results indicated that the long-chain signaling molecule N- 3-oxo-tetradecanoyl-homoserine(3OC14-HSL) could induce resistance to gray mold of tomato, and the resistance effect might be related to the jasmonic acid signal pathway.

Key words: Tomato, N-acyl-homoserine lactone, Botrytis cinerea, Resistance

CLC Number:

S432.4

YANG Xiangyun, LIU Fang, ZHAO Qian, SONG Shuishan, ZHANG Liping. The Exploration of the Effect of N -3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 169-176. doi: 10.7668/hbnxb.20190995.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20190995

http://www.hbnxb.net/EN/Y2020/V35/I4/169

References

[1] Bassler B L,Wright M,Showalter R E,Silverman M R.Intercellular signalling in Vibrio harveyi:sequence and function of genes regulating expression of luminescence[J]. Mol Microbiol,1993, 9(4):773-786.doi:10.1111/j.1365-2958.1993.tb01737.x.
[2] Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria:the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. J Bacteriol, 1994,176(2):269-275.doi:10.1128/jb.176.2.269-275.1994.
[3] Zhao Q, Zhang C, Jia Z H, Huang Y L, Li H L, Song S S. Involvement of calmodulin in regulation of primary root elongation by N -3-oxo-hexanoyl homoserine lactone in Arabidopsis thaliana[J]. Front Plant Sci,2015, 5:807.doi:10.3389/fpls.2014.00807.
[4] Zhao Q, Li M, Jia Z H, Liu F, Ma H, Huang Y L, Song S S.AtMYB44 positively regulates the enhanced elongation of primary roots induced by N -3-oxo-hexanoyl homoserine lactone in Arabidopsis thaliana[J]. Mol Plant Microbe Interact,2016, 29(10):774-785.doi:10.1094/MPMI-03-16-0063-R.
[5] Shenk S T, Schikora A. AHL-priming functions via oxylipin and salicylic acid[J]. Front Plant Sci,2015, 5:784.doi:10.3389/fpls.2014.00784.
[6] 赵芊, 宋水山. N -酰基高丝氨酸内酯调控植物生长发育的研究进展[J]. 植物生理学通讯,2010, 46(10):980-984.doi:10.13592/j.cnki.ppj.2010.10.005. Zhao Q,Song S S. Advances in the regulation of plant growth and development by N -acyl-homoserine lactones[J]. Plant Physiology Newsletter,2010,46(10):980-984.
[7] 赵芊, 贾振华, 宋水山. 细菌信号分子 N -酰基高丝氨酸内酯调控植物抗性的研究进展[J]. 植物生理学报, 2014,50(2):143-149.doi:10.13592/j.cnki.ppj.2014.02.003. Zhao Q,Jia Z H,Song S S. Advances in regulation of plant resistance by N-acyl-homoserine lactones,the bacterial quorum-sensing signals[J]. Plant Physiology Journal, 2014,50(2):143-149.
[8] Oridi M E,Bouarab K. Plant signalling components EDS1 and SGT1 enhance disease caused by the necrotrophic pathogen Botrytis cinerea[J]. New Phytologist,2007,175(1):131-139.doi:10.1111/j.1469-8137.2007.02086.x.
[9] Abramovitch R B,Martin G B. AvrPtoB:a bacterial type Ⅲ effector that both elicits and suppresses programmed cell death associated with plant immunity[J]. Fems Microbiology Letters,2005,245(1):1-8.doi:10.1016/j.femsle.2005.02.025.
[10] Bouarab K,Melton R,Peart J,Baulcombe D,Osboutn A.A saponin-detoxifying enzyme mediates suppression of plant defences[J] .Nature, 2002,418(6900):889-892.doi:10.1038/nature00950.
[11] Chisholm S T,Coaker G,Day B,Staskawicz B J. Host-microbe interactions:shaping the evolution of the plant immune response[J]. Cell, 2006,124(4):803-814.doi:10.1016/j.cell.2006.02.008.
[12] Hann D R,Gimenez-Ibanez S,Rathjen J P.Bacterial virulence effectors and their activities[J]. Current Option in Plant Biology,2010,13(4):388-393.doi:10.1016/j.pbi.2010.04.003.
[13] Janjusevic R,Abramovitch R B,Martin G B,Stebbins C E.A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase[J]. Science,2006,311(5758):222-226.doi:10.1126/science.1120131.
[14] Nomura K, DebRoy S, Lee Y H, Pumplin N, Jones J, He S Y.A bacterial virulence protein supresses host innate immunity to cause plant disease[J]. Science,2006,313(5784):220-223.doi:10.1126/science.1129523.
[15] Rigano L A, Payette C, Brouillard G, Marano M R, Abramowicz L,Torres P S,Yun M, Castagnaro A P, Oirdi E M, Dufour V, Malamud F, Dow J M, Bouarab K,Vojnov A A.Bacterial cyclic β-(1,2)-glucan acts in systemic suppression of plant immune responses[J]. The Plant Cell, 2007,19(6):2077-2089.doi:10.1105/tpc.106.047944.
[16] Yun M H,Torres P S, Oirdi M E, Rigano L A,Gonzalez-Lamothe R, Marano M R,Castagnaro A P,Dankert M A,Bouarab K,Vojnov A A. Xanthan induces plant susceptibility by suppressing callose deposition[J]. Plant Physiology,2006,141(1):178-187.doi:10.1104/pp.105.074542.
[17] Mou Z L, Fan W H, Dong X N. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell,2003,113(7):935-944.doi:10.1016/s0092-8674(03)00429-x.
[18] 邹庆道,张子君,徐明,李海涛.番茄抗灰霉病材料叶、茎和果实抗性的相关性研究[J].园艺学报,2009,36(S1):1972. Zou Q D, Zhang Z J, Xu M, Li H T. Studies on the correlation of leaf, stem and fruit resistance of tomato to gray mold resistance materials[J]. Acta Horticulturae Sinica, 2009, 36 (S1):1972.
[19] 田龙,淡昭菊,关迎池.大棚番茄灰霉病流行规律观察试验[J].湖北植保,2017(2):9-11.doi:10.3969/j.issn.1005-6114.2017.02.005. Tian L, Dan Z J, Guan Y C. Observation and experiment on the epidemic law of tomato gray mold in greenhouses[J]. Hubei Plant Protection, 2017(2):9-11.
[20] Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B. Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction[J]. The Plant Journal,1997,11(6):1187-1194.doi:10.1046/j.1365-313X.1997.11061187.x.
[21] Lamb C, Dixon R A. The oxidative burst in plant disease resistance[J]. Annu Rev Plant Mol Biol,1997,48:251-275.doi:10.1146/annurev.arplant.48.1.251.
[22] Wojtaszek P.Oxidative burst:an early plant response to pathogen infection[J]. Biochem,1997,322(3):681-692.doi:10.1042/bj3220681.
[23] Thoma I, Loeffler C, Sinha A K, Gupta M, Krischke M, Steffan B,Roitsch T, Mueller M J. Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants[J]. The Plant Journal,2003,34(3):363-375.doi:10.1046/j.1365-313x.2003.01730.x.
[24] Hartmann A, Schenk S T, Riedel T, Schröder P, Schikora A.The response of plants toward N -acyl homoserine lactones of quorum-sensing-active bacteria in the rhizosphere[J]. Molecular Microbial Ecology of the Rizosphere 1 & 2,2013,73:77-783.doi:10.1002/9781118297674.ch73.
[25] Schikora A, Schenk S T, Stein E, Molitor A, Zuccaro A, Kogel K H.N-acyl-homoserine lactone confers resistance towards biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6[J]. Plant Physiol, 2011,157(3):1407-1418.doi:10.1104/pp.111.180604.
[26] Schuhegger R, Ihring A, Gantner S, Bahnweg G, Knappe C,Vogg G, Hutzler P, Schmid M, Breusegem F V, Eberl L, Hartmann A, Langebartels C.Induction of systemic resistance in tomato by N -acyl-L-homoserine lactone-producing rhizosphere bacteria[J].Plant Cell Environ, 2006, 29(5):909-918.doi:10.1111/j.1365-3040.2005.01471.x.
[27] Zarkani A A, Stein E, Röhrich C R, Schikora M, Evguenieva-Hackenberg E,Degenkolb T, Vilcinskas A, Klug G, Kogel K H, Schikora A. Homoserine lactones influence the reaction of plants to rhizobia[J]. Int J Mol Sci, 2013,14(8):17122-17146.doi:10.3390/ijms140817122.
[28] Hernández-Reyes C, Schenk S T, Neumann C, Kogel K H,Schikora A.N-acyl-homoserine lactones-producing bacteria protect plants against plant and human pathogens[J]. Microb Biotechnol, 2014,7(6):580-588.doi:10.1111/1751-7915.12177.
[29] Hu Z J,Shao S J,Zheng C F,Sun Z H,Shi J Y,Yu J Q,Qi Z Y,Shi K.Induction of systemic resistance in tomato against Botrytis cinerea by N -decanoyl-homoserine lactone via jasmonic acid signaling[J]. Planta,2018, 247(5):1217-1227.doi:10.1007/s00425-018-2860-7.
[30] Liu F, Zhao Q, Jia Z H, Song C, Huang Y L, Ma H, Song S S. N -3-oxo-octanoyl-homoserine lactone-mediated priming of resistance to Pseudomonas syringae requires the salicylic acid signaling pathway in Arabidopsis thaliana[J]. BMC Plant Biology,2020,20:38.doi:10.1186/s12870-019-2228-6.
[31] Birkennbihl R P, Diezel C, Somssich I E. Arabidopsis WRKY33 is a key transcriptional regulator of hormonal and metabolic responses toward Botrytis cinerea infection[J]. Plant Physiol,2012,159(1):266-285.doi:10.1104/pp.111.192641.
[32] Zabala M D T, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R,Yang R,Truman W,Tang S J, Smirnoff N,Grant M.Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection[J]. New Phytol,2016,209(3):1120-1134.doi:10.1111/nph.13683.
[33] Zhu Z Q, An F Y, Feng Y, Li P P, Xue L, A M, Jiang Z Q, Kim J M, To T K, Li W, Zhang X Y, Yu Q, Dong Z,Chen W Q, Seki M, Zhou J M, Guo H W. Derepression of ethylene-stabilized transcription factors(EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis[J]. Proc Natl Acad Sci,2011,108(30):12539-12544. doi:10.1073/pnas.1103959108.
[34] Zhang S, Li X, Sun Z H, Sun S J, Hu L F,Ye M, Zhou Y H, Xia X J, Yu J Q, Shi K.Antagonism between phytohormone signaling underlies the variation in disease susceptibility of tomato plants under elevated CO₂[J]. Journal of Experimental Botany,2015,66(7):1951-1963.doi:10.1093/jxb/eru538.
[35] Ferrari S, Galletti R, Denoux C, De Lorenzo G, Ausubel F M, Dewdney J.Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid,ethylene,or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3[J]. Plant Physiol,2007,144(1):367-379.doi:10.1104/pp.107.095596.
[36] Rowe H C, Walley J W, Corwin J, Chan E K F, Dehesh K, Kliebenstein D J.Deficiencies in jasmonate-mediated plant defense reveal quantitative variation in Botrytis cinerea pathogenesis[J]. PLoS Pathog,2010,6(4):e1000861.doi:10.1371/journal.ppat.1000861.
[37] Rehman S,Aziz E,Akhtar W,Ilyas M,Mahmood T.Structural and functional characteristics of plant protecinase inhibitor-Ⅱ(PI-Ⅱ)familiy[J]. Biotechnol Lett,2017,39(5):647-666.doi:10.1007/s10529-017-2298-1.

Please choose a citation manager

Content to export