Study on Transformation in Maize of Pollen Lethal Gene ZmAA1

doi:10.7668/hbnxb.2016.03.015

Abstract

Abstract: In order to study the function of pollen lethal gene ZmAA1 and create tansgenic male sterile materials. ZmAA1 and its specific promoter Pg47 were found according to GenBank,designed to plus restriction endonuclease on the downstream and inserted them into an cloning vector puc57,construction of vector pCAMBIA3300-Pg47-ZmAA1-35S-bar used traditional construction methods digested connection.A maize inbred line Zheng 58 was infected by Agrobacterium tumefacfaciens with the vector pCAMBIA3300-Pg47-ZmAA1-35S-bar, 94 PPT-resistant seedlings were obtained and 47 plants were PCR positive.The pollen sterility in greenhouse RT-PCR assay for gene bar in the transgenic plant leaves and immuno strip test suggested that pollen lethal gene ZmAA1 had been integrated into the maize genome,and protein was expressed.

Key words: Maize, ZmAA1 gene, Pg47 promoter, Genetic transformation

CLC Number:

S513.03

MENG Lingcong, SONG Guangshu, LÜ Qingxue, LIU Hongwei, ZHANG Zhijun, LI Chunlei, WANG Min, LIU Wenguo. Study on Transformation in Maize of Pollen Lethal Gene ZmAA1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 101-106. doi: 10.7668/hbnxb.2016.03.015.

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http://www.hbnxb.net/EN/Y2016/V31/I3/101

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