Mapping of QTLs for Bud-stage Salinity Tolerance Based on Chromosome Segment Substitution Line in Rice

doi:10.7668/hbnxb.2017.05.016

Abstract

Abstract: The identification of salt tolerant genetic loci in rice can provide study basis for the molecular mechanism of salt tolerance,and gene resources for improving salt tolerant cultivars.A set of chromosome segment substitution lines (CSSLs) developed from two sequenced rice cultivars:the indica variety 9311 as the recipient and the japonica variety Nipponbare as a donor were used to detect for salt tolerance in bud stage quickly,and to locate QTLs related to salt tolerance.9 CSSLs showed significant salt tolerance and 4 QTLs were mapped by genetic background detection based on high density molecular markers and the substitution mapping.These 4 QTLs located on rice chromosomes 1,2,4 and 11 chromosomes,designated as qSAT1, qSAT2, qSAT4 and qSAT11. qSAT1 was detected in 4 CSSLs containing over lapping fragments,while the other 3 QTLs were detected in 2 CSSLs.These QTLs were not included in the same chromosome interval as the cloned rice salt tolerance genes,and thus were described as new candidate gene loci associated with bud-stage salt tolerance.The results supplies important information for further exploration and utilization of new salt tolerant QTL in rice.

Key words: Oryza sativa L., Bud stage, Salt tolerance, QTL

CLC Number:

S511.03

ZHAO Chunfang, ZHANG Shanlei, ZHAO Qingyong, ZHOU Lihui, ZHAO Ling, YAO Shu, ZHANG Yadong, WANG Cailin. Mapping of QTLs for Bud-stage Salinity Tolerance Based on Chromosome Segment Substitution Line in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 106-111. doi: 10.7668/hbnxb.2017.05.016.

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