Cloning,Bioinformatics and Expression Analysis of TaWRKY71a Gene in Wheat

doi:10.7668/hbnxb.2018.03.002

Abstract

Abstract: WRKY proteins are a large super family of transcription factors(TFs)and exist mainly in plants. It has been well documented that WRKY TFs play important roles in plant growth and development,and responses to stresses. In order to deeply understand the gene function of WRKY,a novel WRKY gene,named TaWRKY71a, was isolated from common wheat. The coding sequence of this gene was predicted with the bioinformatics analysis. Furthermore,qRT-PCR assay was performed to investigate the tissue-specific expression of TaWRKY71a,and its expressed patterns under various abiotic stresses and signaling molecules treatments. The results showed that TaWRKY71a gene encoded a protein with 355 amino acid residues. Sequence analysis showed that TaWRKY71a contained one WRKY domain and C₂H₂ zinc-finger structure. Based on bioinformatics analysis,TaWRKY71a was located in the nucleus. The potential secondary structure for the protein included about 28.17% alpha helixes,16.34% extended strand,4.79% beta turn and 50.70% random coil as predicted with softwares. The phylogenic tree indicated that TaWRKY71a shared high similarity to WRKY71 from Aegilops tauschii, Oryza sativa, Sorghum bicolor and WRKY40 from Arabidopsis. TaWRKY71a expressed differentially in various organs such as leaves,roots,stems,flowers and seeds of wheat. After treatment with ABA,NaCl and PEG, TaWRKY71a showed higher expression levels than control. The fact implied that it might be involved in stress signaling pathways. The results will provide a basis for further functional studies of TaWRKY71a.

Key words: Wheat, TaWRKY71a gene, Cloning, Gene expression, Bioinformatics

CLC Number:

Q785
S512.03

WANG Junbin, YANG Wenli, DING Bo, WU Tianwen, WANG Haifeng, XIE Xiaodong. Cloning,Bioinformatics and Expression Analysis of TaWRKY71a Gene in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 7-13. doi: 10.7668/hbnxb.2018.03.002.

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