Bioinformatics Analysis of GmbHLH041 of Soybean and Its Response to Cadmium Stress

doi:10.7668/hbnxb.2018.03.003

Abstract

Abstract: In order to investigate the roles of GmbHLH041 in soybean response to cadmium stress, GmbHLH041 was chosen based on the primary transcriptome sequencing of soybean roots after cadmium treatment. By using NCBI,phytozome database combined with bioinformatics software,the systematic prediction and analysis of GmbHLH041 were conducted,and by qRT-PCR,the expression of GmbHLH041 in soybean roots was investigated after 50 μmol/L CdSO₄ treatment for 0 and 24 h. GmbHLH041 existed mainly in Random coil(39.48%),Alpha helix(37.27%)and Extended strand(16.61%)according to the secondary structure prediction. The results showed that GmbHLH041 encoded 542 amino acids,the relative molecular weight was 61.17 ku and the theoretical pI was 5.89. Phylogenetic analysis of GmbHLH041 was conduncted with 24 bHLH protein from 12 subgroup of bHLH family in Arabidopsis thaliana and other closely related bHLH proteins of other species. The results of phylogenetic analysis showed that the GmbHLH041 protein had a close homologous relationship with GsbHLH041 and LabHLH041 and belonged to the subclass Ⅳd of Arabidopsis thaliana bHLH proteins. Acording to the results of phylogenetic analysis,alignment of GmbHLH041 with closely related GsbHLH041,LabHLH041 and AT5G56960 from the bHLH subclass Ⅳd of Arabidopsis thaliana,the results showed that GmbHLH041 had a conserved bHLH domain. The expression of GmbHLH041 was up-regulated significantly after 50 μmol/L CdSO₄ treatment for 24 h,indicating its important function in response to cadmium stress.

Key words: Soybean, Cadmium, bHLH

CLC Number:

Q78
S565.01

LIU Xiaoqing, CHEN Huatao, ZHANG Hongmei, YUAN Xingxing, CUI Xiaoyan, CHEN Xin. Bioinformatics Analysis of GmbHLH041 of Soybean and Its Response to Cadmium Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 14-18. doi: 10.7668/hbnxb.2018.03.003.

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