Abstract: To reveal pollen viability in jasmine and provide an efficient method for the detecting,the effects of sampling stage,medium type,concentrations of different additions,pH value,culture temperature,light intensity and time on the pollen germination and pollen tube growth were investigated.Meanwhile,the method was identified further through observing the pollen germinated on stigmas under scanning electron microscopy.The results showed that pollen of the highest viability should be collected at 8:00-9:00 am,the optimal medium was BK(Containing H₃BO₃ 100 mg/L,KNO₃ 100 mg/L,MgSO₄·7H₂O 200 mg/L and Ca(NO₃)₂·4H₂O 300 mg/L)added with sucrose 80 g/L and PEG4000 120 g/L,with the suitable pH value of 6.0,temperature of 25-30 ℃,light intensity of 35-50 μmol/(m²·s)and culture time of 3-4 h.Correlation analysis showed that pollen germination and pollen tube growth were very significantly positively correlated with the indices of light intensity and culture time(P<0.01),positively with the pH value,negatively with the concentrations of sugar and PEG4000,and negatively and positively with the temperature,respectively,at no significant levels(P>0.05).The research shows that jasmine pollen has a low viability on the whole,and germination in vivo was indeed a simple,quick and reliable method to determine the viability.But much attention should be paid to the selection and optimization of culture conditions to obtain the best effectiveness.

Key words: Jasmine, Pollen viability, Pollen culture, Pollen tube growth, Scanning electron microscopy

CLC Number: 

• S685.03