Analysis on Polymorphism of CALCA Gene in Zaosheng Cattle and Zaosheng Hybrids Cattle and Their Association with Carcass Trait

doi:10.7668/hbnxb.2014.05.019

Abstract

Abstract: The aim was to analyze association of CALCA gene polymorphisms with some economic traits in Zaosheng cattle and Zaosheng crossbred,and to evaluate it as a candidate molecular marker associated with beef quality.PCR-SSCP method was used to detect the genetic variation inCALCA gene in 362 cattle from 5 cattle groups,including Zaosheng in Qingyang Prefecture,Zaosheng cattle in Pingliang Prefecture,Nanza(South Devon cattle×Qingyang groups),Qinza(Qinchuan cattle×Pingliang groups),Xiza(Simmental×Pingliang groups).The association between SNP locus and some carcass traits of Pingliang groups were analyzed.Two genotypes AA and AB were detected at exon 4 in CALCA gene.Analysis of association of polymorphism with carcass traits at mutation locus showed that there were significant effects on WBSF,Ribeye area,Hot carcass weight and Cooking loss(P<0.05)and there was no effect on other traits(P>0.05).In conclusion,SNP of CALCA gene could be a genetic mark for carcass trait.

Key words: Beef cattle, CALCA gene, SNP, Analysis of population genetic variations, Carcass traits

CLC Number:

S823

ZHANG Cai-xia, ZHAO Sheng-guo, LI Tian-ke, LEI Zhao-min. Analysis on Polymorphism of CALCA Gene in Zaosheng Cattle and Zaosheng Hybrids Cattle and Their Association with Carcass Trait[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 114-118. doi: 10.7668/hbnxb.2014.05.019.

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