Genetic Diversity Analysis of Wheat Varieties with Different Aphids-resistant by Simple Sequence Repeat(SSR) Markers

doi:10.7668/hbnxb.2014.05.020

Abstract

Abstract: In order to explore the genetic diversity of wheat varieties with different aphid-resistant degrees,and then offer the reference for breeding new aphid-resistant cultivars,43 wheat varieties/lines with different aphid-resistant degrees,based on a two-year field appraisal results for aphid index by nature aphid populations,were studied by Simple sequence repeat(SSR)markers.The results showed that fifty-four markers were screened and a total of 365 allelic variants were detected in these 43 varieties/lines,ranging from 2 to 18 alleles per marker with an average of 6.7. The PIC(Polymorphism information contents)for each polymorphic primer varied from 0.05-0.91,with an average of 0.65.The mean genetic distance(GD)among 43 varieties was 0.52,varying from 0.30-0.90.Based on the 0.55 of GD 43 varieties were clustered into five groups.Resistant insect variety Jingdong 6 was a group by oneself.There was a further genetic relationship between Jingdong 6 and other varieties,and so the Jingdong 6 might be used as a resistant insect parent.Therefore,SSR analysis could reliable determine genetic relationships within a diverse array of wheat varieties and effectively provide the data for selecting parents for breeding programme.

Key words: Wheat, Aphid, Simple sequence repeats(SSR), Genetic diversity

CLC Number:

XU Lan-jie, HOU Qi-ling, HOU Chun-yu, ZHANG Ting, ZHANG Xiao-hua, NI Zhong-fu, LIANG Rong-qi. Genetic Diversity Analysis of Wheat Varieties with Different Aphids-resistant by Simple Sequence Repeat(SSR) Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 119-124. doi: 10.7668/hbnxb.2014.05.020.

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