Identification and Classification of Different Male Sterile Cytoplasms in Radish

doi:10.7668/hbnxb.2014.05.021

Abstract

Abstract: The classification of different male sterile cytoplasms can provide guide to the utilization of it.In this study,we have investigated five cytoplasm male steriles(CMS)of radish.The five cytoplasm male sterile lines were crossed with 8 fertile breeding lines,and the fertility of the F₁ progenies was different.According to the fertility of the F₁ progenies,the five cytoplasms were classified into two types.Cytological examination of pollen development stages showed a clear difference in the defective stage from that seen in the two types of male sterility.At the tetrad stage,the tapetum cells of type 1 CMS was bulged by severe cytoplasmic vacuolation,but no significant differences were observed in type 2 CMS during this stage.After the microspores were released,microspores of the type 1 CMS began to degenerate finally resulting in empty anthers without pollen grains.However,the microspores of the type 2 CMS plant failed to develop into mature pollen grains.The size of the just-released microspores and the just-before-dehiscence stage microspores remained unchanged.PCR analysis revealed that the type 1 was Ogura CMS and the other was NWB CMS.

Key words: Raphanus sativus L., Male sterile cytoplasm, Classification

CLC Number:

Q631.03

ZHANG Li, WANG Qing-biao, ZHENG Peng-jing. Identification and Classification of Different Male Sterile Cytoplasms in Radish[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 125-129. doi: 10.7668/hbnxb.2014.05.021.

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