Establishment of Subspecies Classification of Indica and Japonica System by SSR Markers

doi:10.7668/hbnxb.2009.01.027

Abstract

Abstract: A recombinant inbred line(R1L)population of F8 generations derived from the cross between qshanzhan(typical indica) and Akihikari (typical japonica) were used as materials. All of 122 SSR markers containing 94 SSR markers which was distributed on 12 chromosome of rice and 28 indica and japonica specific SSR markers were used to markeranalysis. Based on QTL analysis of Cheng's index,34 markers linked to Cheng's index were found. The 34 markers and 28indica and japonica specific SSR markers were conducted to determine the blood relationship of indica and japonica on thepopulation derived from indica and japonica crossing,and to study the effect of markers types on subspecies classificationof indica and japonica. 3,5,8 SSR markers from per chromo summed to 34,65,90 SSR markers were evenly selectedto determine blood relationship of indica and japonica on the population and to study the effect of markers amount on sub-species classification of indica and japonica. The results showed that the distribution of population derived from indica andjaponica crossing were almost continuous distribution keng (japonica) subspecies and japonica. Indica and hsien (indica)were minority. The consistent degree of classification based on the 28 indica and japonica specific SSR markers and 34markers or all of 122 SSR markers were 96%and 90%by the limit of 50%blood relationship of indica and japonica.The consistent degree of classification based on 34,65,90 markers were 91%,97%and 99%.The consistent degree ofclassification based on 28 indica and japonica specific SSR markers and 65 markers or 122 markers were more than 95%.Using few SSR markers can analyse blood relationship of indica and japopica on the population derived from indica and japomca crossing.

Key words: Rice, Recombinant inbred line, Classification, SSR markers, Markers type, Markers amount

CLC Number:

S511.2

MAO Ting, XU Hai, GUO Yan-hua, ZHU Chun-jie, CHEN Kai, WANG Jia-yu, XU Zheng-jin. Establishment of Subspecies Classification of Indica and Japonica System by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 119-124. doi: 10.7668/hbnxb.2009.01.027.

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